Overview

  • Product name

    Human BHMT Matched Antibody Pair Kit
    See all BHMT kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human BHMT.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab221830) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human BHMT Capture Antibody 1 x 100µg 1 x 20µg
    Human BHMT Detector Antibody 1 x 25µg 1 x 5µg
    Human BHMT Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  • Tissue specificity

    Found exclusively in liver and kidney.
  • Pathway

    Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
    Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
  • Sequence similarities

    Contains 1 Hcy-binding domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • Betaine homocysteine methyltransferase
    • Betaine homocysteine S methyltransferase
    • Betaine homocysteine S methyltransferase 1
    • Betaine--homocysteine S-methyltransferase 1
    • BHMT
    • BHMT1
    • BHMT1_HUMAN
    • EC 2.1.1.5
    • Epididymis secretory sperm binding protein Li 61p
    • HEL S 61p
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab222261 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222261.
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