Human BMP2 ELISA Kit (ab119581)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BMP2 ELISA Kit
See all BMP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 55pg/ml 4.29 = 7.8% 2 16 259pg/ml 13.25 = 5.3% 3 16 1005ng/ml 70.35 = 7% Inter-assay Sample n Mean SD CV% 1 24 59pg/ml 4.66 = 7.9% 2 24 255pg/ml 15.55 = 6.1% 3 24 1061pg/ml 83.81 = 7.9% -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human BMP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BMP2 in bone tissue, cell culture supernatants and serum.
A BMP2 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection monoclonal antibody from mouse, specific for BMP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BMP2 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human BMP2 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- human BMP2 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant human BMP2 standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Induces cartilage and bone formation. -
Tissue specificity
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BDA2
- BMP-2
- BMP-2A
see all -
Database links
- Entrez Gene: 650 Human
- Omim: 112261 Human
- SwissProt: P12643 Human
- Unigene: 73853 Human
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab119581 has been referenced in 11 publications.
- E L et al. Microenvironment Influences on Human Umbilical Cord Mesenchymal Stem Cell-Based Bone Regeneration. Stem Cells Int 2021:4465022 (2021). PubMed: 34447439
- Chen Z et al. Fabricating a novel HLC-hBMP2 fusion protein for the treatment of bone defects. J Control Release 329:270-285 (2021). PubMed: 33278483
- Lee SS et al. A Biomimetic Macroporous Hybrid Scaffold with Sustained Drug Delivery for Enhanced Bone Regeneration. Biomacromolecules 22:2460-2471 (2021). PubMed: 33971092
- Xie S et al. Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling. Int J Biol Sci 17:2367-2379 (2021). PubMed: 34239363
- Liu F et al. USF2 enhances the osteogenic differentiation of PDLCs by promoting ATF4 transcriptional activities. J Periodontal Res 55:68-76 (2020). PubMed: 31448831