Human BMP2 ELISA Kit (ab277085)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 79.8 pg/ml
- Range: 187.5 pg/ml - 12000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human BMP2 ELISA Kit
See all BMP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.7% Inter-assay Sample n Mean SD CV% Serum 3 4.2% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
79.8 pg/ml -
Range
187.5 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 97 92% - 107% Serum 101 99% - 105% Cell culture media 99 96% - 102% Hep Plasma 95 90% - 101% EDTA Plasma 107 97% - 107% Cit plasma 95 91% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BMP2 ELISA kit (ab277085) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BMP2 protein in human serum, plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMP2 with 79.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Bone morphogenetic protein-2 (BMP2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP2 has been identified as a candidate mediator of retinoid activity. BMP2 protein induces medulloblastoma cell apoptosis, whereas the BMP2 antagonist noggin blocks both retinoid and BMP2-induced apoptosis. BMP2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BMP2 Capture Antibody 1 x 600µl 10X Human BMP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human BMP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Induces cartilage and bone formation. -
Tissue specificity
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- BDA2
- BMP-2
- BMP-2A
see all -
Database links
- Entrez Gene: 650 Human
- Omim: 112261 Human
- SwissProt: P12643 Human
- Unigene: 73853 Human
Associated products
-
Alternative Versions
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human BMP2 standard curve in Sample Diluent NS.The BMP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
. Interpolated concentrations of spike BMP2 in human serum, plasma and cell culture media samples.The concentrations of BMP2 were measured in duplicates, interpolated from the BMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Sandwich ELISA - Human BMP2 ELISA KitTo learn more about the advantages of recombinant antibodies see here.
References (0)
ab277085 has not yet been referenced specifically in any publications.
