Overview

  • Product name

    Human BMP4 ELISA Kit
    See all BMP4 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    < 96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.25 85% - 104%
    Serum 92.95 80% - 101%
    Plasma 94.32 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s BMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab99982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99982.

  • Representative Standard Curve using ab99982.

Protocols

References

This product has been referenced in:

  • Eslaminejad F  et al. BMP4 circulating levels and promoter (rs17563) polymorphism in risk prediction of idiopathic male infertility. Br J Biomed Sci N/A:1-3 (2019). Read more (PubMed: 30628543) »
  • Wang X  et al. New association of bone morphogenetic protein 4 concentrations with fat distribution in obesity and Exenatide intervention on it. Lipids Health Dis 16:70 (2017). ELISA ; Human . Read more (PubMed: 28376799) »
See all 3 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. Both kits ab99982 and ab99983 use the same antibody pairs. The homology between mouse and human BMP4 is 98% and it is therefore likely that these kits will detect mouse BMP4 as well. This has not been tested in house however, and results on mouse samples would not be guaranteed. I hope this helps, please let me know if you need any additional information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up