Product nameHuman BMP4 ELISA Kit
See all BMP4 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 15 pg/ml
Range8.23 pg/ml - 6000 pg/ml
< 96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.25 85% - 104% Serum 92.95 80% - 101% Plasma 94.32 82% - 102%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s BMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from
Optimization may be required with urine samples.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-human BMP-4 2 vials BMP4 Microplate (12 strips x 8 wells) 1 unit BMP4 Standard Human (recombinant) (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceBMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.
- BMP 2B
- BMP 4
- SwissProt: P12644 Human
Our Abpromise guarantee covers the use of ab99982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Eslaminejad F et al. BMP4 circulating levels and promoter (rs17563) polymorphism in risk prediction of idiopathic male infertility. Br J Biomed Sci N/A:1-3 (2019). Read more (PubMed: 30628543) »
- Wang X et al. New association of bone morphogenetic protein 4 concentrations with fat distribution in obesity and Exenatide intervention on it. Lipids Health Dis 16:70 (2017). ELISA ; Human . Read more (PubMed: 28376799) »