Overview

  • Product name

    Human BMP4 ELISA Kit
    See all BMP4 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    91 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 92.45 80% - 103%
    Tissue Extracts 89.47 78% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human BMP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP4 in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab99983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99983

Protocols

References

ab99983 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

DISCOUNT CODE: ABTRIAL-xxxxx
Expiration date: 2013-03-01
Value: 490 euro

I am very pleased to hear you would like to accept our offer and test the BMP4 Human ELISA Kit (ab99983) on cerebrospinal fluid. This code will give you 490.00 euro off your next order before the expiration date. To redeem this offer, please submit an Abreview with an image for cerebrospinal fluid tested with the BMP4 Human ELISA Kit (ab99983) and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:


1) Call to place your order and mention the code to our customer service department;

2) Include the code in your fax order;

3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for contacting us.

I got feedback from the lab regarding your inquiry about the BMP4 ELISA kit (ab99983). I can confirm that there are no specific references testing cerebrospinal fluid (CSF) on the BMP4 ELISA kit. However, the ELISA kits is very accommodating to most body fluids and it is likely to work well with CSF samples. For the reason that secreted proteins usually at fairly low levels in CSF samples we would recommend to consider starting with a minimal dilution such as neat (no dilution) or 2-fold.

I would like to offer you to participate in our AbTrial program. By doing this you are able to use our products in an untested application or species/sample without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results and an image, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/index.html?pageconfig=resource&rid=11998&source=pagetrap&viapagetrap=abtrial.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your inquiry.

Epitope mapping has not been performed so we do not know exactly where the antibodies are binding the BMP-4 protein. All we know is that they are binding somewhere between aa 293-408 (which looks to be the entire mature protein sequence). The immunogen was a NS0-derived recombinant human BMP-4.Finally, both the capture and detection antibody are mouse monoclonal.

I hope this information is helfpul. Please do not hesitate to contact us agains hould you have any further questions.

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Answer

Thank you for contacting us. The sensitivity of this kit is less than 15 picogram/ml. This kit can be successfully used to detect BMP4 at a concentration range between 8.23 - 6000 pg/ml. We unfortunately have not received any citation information from our customers. So unfortunately we cannot provide any publication reading the use of this kit. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us. Both kits ab99982 and ab99983 use the same antibody pairs. The homology between mouse and human BMP4 is 98% and it is therefore likely that these kits will detect mouse BMP4 as well. This has not been tested in house however, and results on mouse samples would not be guaranteed. I hope this helps, please let me know if you need any additional information.

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