Human Bone Metabolism Antibody Array (31 Targets) - Quantitative (ab197423)


  • Product name

    Human Bone Metabolism Antibody Array (31 Targets) - Quantitative
    See all Bone Metabolism Antibody Array antibody arrays
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Assay type

  • Assay time

    6h 00m
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab197423 can be used for quantitative measurement of 31 Human Bone Metabolism proteins. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
    Targets: Activin A, aFGF, AR, bFGF, BMP-4, BMP-9, E-Selectin, ICAM-1, IGF-1, IL-1α, IL-1β, IL-6, IL-8, IL-11, IL-17, MCP-1, M-CSF, MIP-1α, MMP-2, MMP-9, MMP-13, Osteoactivin, P-Cadherin, RANK, SDF-1α, ShhN, TGFβ1, TGFβ2, TNFα, VCAM-1, VE-Cadherin. 

    Learn more about Quantitative antibody arrays

  • Notes

    Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. 

    Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. 

    For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. 

    Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. 

    Learn more about quantitative antibody arrays 

    Learn more about membrane antibody arrays


  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 8 tests 22 tests 50 tests
    20X Wash Buffer I 2 x 30ml 3 x 30ml 6 x 30ml
    20X Wash Buffer II 1 x 30ml 1 x 30ml 2 x 30ml
    Cy3 equivalent dye-conjugated Streptavidin 1 x 5µl 2 x 5µl 4 x 5µl
    Human Bone Metabolism Array (31 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units
    Human Bone Metabolism Array (31 T) Glass Slide 1 slide 2 slides 4 slides
    Human Bone Metabolism Array (31 T) Lyophilized Cytokine Standard Mix 1 vial 2 vials 2 vials
    Sample Diluent 1 x 15ml 1 x 15ml 2 x 15ml
    Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 1 unit 2 units


  • Assay summary for Abcam Human Bone Metabolism Antibody Array (31 Targets) - Quantitative.

  • Array Map for Abcam Human Bone Metabolism Antibody Array (31 Targets) - Quantitative (ab197423).

  • Standard Curve obtained using Abcam Human Bone Metabolism Antibody Array (31 Targets) - Quantitative (ab197423).



ab197423 has not yet been referenced specifically in any publications.

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