Product nameHuman Anti-Bordetella pertussis IgG ELISA Kit
See all Bordetella pertussis IgG kits
Intra-assay Sample n Mean SD CV% Pos. Serum 20 3.9% Pos. Serum 24 5.2% Inter-assay Sample n Mean SD CV% Pos. Serum 12 4.9% Pos. Serum 12 6.1%
Sample typeSerum, Heparin Plasma, Citrate Plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Bordetella pertussis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Bordetella pertussis in Human serum and plasma.
A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgG sample captured in plate.
Tested applicationsSuitable for: Indirect ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Bordetella pertussis (IgG) Coated Microplate (12 x 8 wells) 1 unit Bordetella pertussis anti-IgG HRP Conjugate Black cap 1 x 20ml Bordetella pertussis IgG Cut-off Control Green cap 1 x 3ml Bordetella pertussis IgG Negative Control Blue cap 1 x 2ml Bordetella pertussis IgG Positive Control Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
Our Abpromise guarantee covers the use of ab108709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108709 has not yet been referenced specifically in any publications.