Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Brucella IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos.Serum 23 5.3% Pos.Serum 24 4.2% Inter-assay Sample n Mean SD CV% Pos.Serum 12 3.6% Pos.Serum 12 4.8%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.
A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Brucella (antigen) Coated Microplate (12 x 8 wells) 12 Strips of 8 wells 1 unit Brucella anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Brucella IgG Cut-off Control Yellow, Green cap 1 x 3ml Brucella IgG Negative Control Yellow, Blue cap 1 x 2ml Brucella IgG Positive Control Yellow, Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- B. abortus IgG
- B. canis IgG
- B. melitensis IgG
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108712 has been referenced in 1 publication.
- Zhu J et al. Lysine methylation represses p53 activity in teratocarcinoma cancer cells. Proc Natl Acad Sci U S A 113:9822-7 (2016). WB ; Human . PubMed: 27535933