Human BSG ELISA Kit (CD147) (ab119592)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameHuman BSG ELISA Kit (CD147)
See all CD147 kits -
Detection methodColorimetric
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Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 2 pg/ml
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Range31.2 pg/ml - 2000 pg/ml
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Human BSG (CD147) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BSG in cell culture supernatants, serum, (heparin, EDTA), saliva, urine and Human milk.
A BSG specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BSG is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BSG amount of sample captured in plate.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests ABC Diluent Buffer 1 x 12ml Antibody Diluent Buffer 1 x 12ml Anti-Human BSG antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human BSG antibody 1 x 130µl Lyophilized recombinant Human BSG standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer 1 x 30ml TMB Color Developing Agent 1 x 10ml TMB Stop Solution 1 x 10ml -
Research areas
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FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
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Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
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Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Alternative names
- 5F7
- BASI_HUMAN
- Basigin
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Database links
- Entrez Gene: 682 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
Applications
Our Abpromise guarantee covers the use of ab119592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab119592
Protocols
References
ab119592 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"