Human BSG ELISA Kit (CD147) (ab119592)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human BSG ELISA Kit (CD147)
See all CD147 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human BSG (CD147) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BSG in cell culture supernatants, serum, (heparin, EDTA), saliva, urine and Human milk.
A BSG specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BSG is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BSG amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human BSG antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human BSG antibody 1 x 130µl Lyophilized recombinant Human BSG standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- 5F7
- BASI_HUMAN
- Basigin
see all -
Database links
- Entrez Gene: 682 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
Images
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Typical Standard CurveRepresentative Standard Curve using ab119592
Protocols
References
This product has been referenced in:
- Abu El-Asrar AM et al. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a potential biomarker of angiogenesis in proliferative diabetic retinopathy. Acta Ophthalmol 95:697-704 (2017). Read more (PubMed: 27860331) »
