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Immunology Cell Type Markers CD Endothelial Cells
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Human BSG ELISA Kit (CD147) (ab119592)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 2 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Recombinant Human CD147 protein (Fc Chimera His Tag) (ab229371)

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    Overview

    • Product name

      Human BSG ELISA Kit (CD147)
      See all CD147 kits
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 2 pg/ml
    • Range

      31.2 pg/ml - 2000 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Human BSG (CD147) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BSG in cell culture supernatants, serum, (heparin, EDTA), saliva, urine and Human milk.

      A BSG specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BSG is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BSG amount of sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human BSG antibody Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human BSG antibody 1 x 100µl
      Lyophilized recombinant Human BSG standard 2 x 10ng
      Plate Seal 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Endothelial Cells
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Neuroscience
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      • Neurogenesis
      • Microbiology
      • Organism
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      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MMPs
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      • Invasion/microenvironment
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      • Other
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Kits/ Lysates/ Other
      • Kits
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      • ELISA Kits
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      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
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      • Types of disease
      • Cancer
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers
    • Function

      Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
    • Tissue specificity

      Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P35613 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • 5A11 antigen
      • 5F7
      • BASI_HUMAN
      • Basigin
      • Basigin (Ok blood group)
      • Blood brain barrier HT7 antigen
      • Bsg
      • CD 147
      • CD147
      • CD147 antigen
      • Collagenase stimulatory factor
      • EMMPRIN
      • Extracellular matrix metalloproteinase inducer
      • Leukocyte activation antigen M6
      • M 6
      • M6
      • M6 leukocyte activation antigen
      • Neurothelin
      • OK
      • OK blood group
      • OK blood group antigen
      • TCSF
      • Tumor cell derived collagenase stimulatory factor
      • Tumor cell-derived collagenase stimulatory factor
      see all
    • Database links

      • Entrez Gene: 682 Human
      • Omim: 109480 Human
      • SwissProt: P35613 Human
      • Unigene: 501293 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab119592

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (1)

        Publishing research using ab119592? Please let us know so that we can cite the reference in this datasheet.

        ab119592 has been referenced in 1 publication.

        • Abu El-Asrar AM  et al. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a potential biomarker of angiogenesis in proliferative diabetic retinopathy. Acta Ophthalmol 95:697-704 (2017). PubMed: 27860331

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