Overview

  • Product name

    Human BST2 ELISA Kit
    See all BST2/Tetherin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8.2%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Range

    5 pg/ml - 300 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101 99% - 103%
    Cell culture extracts 113 109% - 117%
    Cell culture media 100 99% - 100%
    Hep Plasma 96 93% - 1010%
    EDTA Plasma 102 99% - 104%
    Cit plasma 100 98% - 103%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    BST2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BST2 protein in human serum, plasma, cell culture supernatants, and cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Bone Marrow Stromal Antigen 2, or BST2, also known as Tetherin, HM1.24 antigen, and CD317 is a protein encoded by the BST2 gene. BST2 is a type 2 transmembrane, lipid raft associated protein and is expressed by the IFN-dependent antiviral response pathway as part of the antiviral host restriction factors. BST2 acts by tethering nascent virions to the membranes of infected cells, subsequently limiting diffusion of the budding virions. BST2 is a cysteine linked homodimer on the surface of cells, which dimerization has been demonstrated to be essential for its antiviral activity.


    Sensitivity:


    Samples diluted in Sample Diluent NS = 0.28 pg/ml.


    Samples diluted in 1X Cell Extraction Buffer PTR = 0.25 pg/ml.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human BST2 Capture Antibody 1 x 600µl
    10X Human BST2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Human BST2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • Tissue specificity

    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • Sequence similarities

    Belongs to the tetherin family.
  • Domain

    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • Post-translational
    modifications

    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • Cellular localization

    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Information by UniProt
  • Alternative names

    • Bone marrow stromal antigen 2
    • Bone marrow stromal cell antigen
    • Bone marrow stromal cell antigen 2
    • BST 2
    • BST-2
    • BST2
    • BST2_HUMAN
    • CD 317
    • CD317
    • CD317 antigen
    • HM1.24 antigen
    • NPC A 7
    • Tetherin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab231931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The BST2 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of BST2 were measured in duplicates, interpolated from the BST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (heparin) 100%, plasma (EDTA) 50%, and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration in the neat samples was determined to be 172 pg/mL in serum, 148 pg/mL in plasma (heparin), 133 pg/mL in plasma (EDTA), and 206 pg/mL in plasma (citrate).

  • The concentrations of BST2 were measured in duplicate and interpolated from the BST2 standard curve and corrected for sample dilution. Undiluted samples are as follows: RPMI media 50%, A431 cell extract 10 µg/mL, and HeLa cell extract 30 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration was determined to be 221 pg/mL with a range of 74 – 672 pg/mL.

  • The BST2 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab231931 has not yet been referenced specifically in any publications.

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