Associated products


Our Abpromise guarantee covers the use of ab226013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Bub3 antibody [EPR5319(2)] (ab133699)

  • Form

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.

    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.

    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.

    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.

    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

General Info

  • Alternative names

    • Aa2-050
    • AU019800
    • AU021329
    • AU043350
    • AW146323
    • BUB 3
    • BUB 3L
    • bub3
    • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
    • BUB3 budding uninhibited by benzimidazoles 3 homolog
    • BUB3 mitotic checkpoint protein
    • BUB3_HUMAN
    • BUB3L
    • Budding uninhibited by benomyl
    • Budding uninhibited by benzimidazoles 3
    • Budding uninhibited by benzimidazoles 3 homolog
    • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae)
    • Budding uninhibited by benzimidazoles 3 homolog (yeast)
    • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of
    • C78067
    • hBUB3
    • Mitotic checkpoint component
    • Mitotic checkpoint protein bub3
    • OTTHUMP00000020679
    • OTTHUMP00000020680
    • WD repeat type I transmembrane protein A72.5
    see all
  • Function

    Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
  • Sequence similarities

    Belongs to the WD repeat BUB3 family.
    Contains 5 WD repeats.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
  • Information by UniProt


ab226013 has not yet been referenced specifically in any publications.

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