Description

Associated products

Specifications

Our Abpromise guarantee covers the use of ab93903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-C Peptide antibody (ab14181)

  • Form

    Lyophilised
  • Additional notes

    C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

    This peptide can be used with studies using ab14181.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Reconstitution
    Resuspend in 0.1% acetic acid for required concentration

General Info

  • Alternative names

    • IDDM 2
    • IDDM2
    • ILPR
    • ins
    • INS_HUMAN
    • Insulin A chain
    • IRDN
    • MODY10
    • Proinsulin
    see all
  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab93903 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I agree the antibodies should detect the proteins in serum; as this is not the case so I am happy to replace these products. Would you like try new vials of these antibodies or a different antibody against the same target?
My suggestion would be trying one antibody first and if you get satisfactory results then I will send you the second.
Though ab93903 has not been tested in ELISA with ab8297 and ab9298 however lab has confirmed using this in titre test. The following procedure they used; maybe this information will help!
1 µg/ml with carbonat-buffer, NaHCO3, pH 9.6, for 2 hours by room-temperature,

100µl each well for coating.
Could you confirm how you would like to proceed?

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Question

Thanks for replying so promptly. See below for answers to your questions:

Q. Could you provide the source of protein?. Is it possible if you could provide sequence of C-peptide used?
A. We are using abcam's C-peptide calibrator (ab93903). The sequence is EAEDLQVGQV ELGGGPGAGS LQPLALEGSL Q, corresponding to amino acids 57-87 of Human C Peptide
The detection antibody recognizes the same sequence only it's conjugated to Biotin. The immunogen for the capture Ab (ab8297) is the recombinant full length protein.

Q. What was the secondary antibody used? Does this antibody worked before?
We are using an anti-mouse antibody from meso scale discovery. It's a goat polyclonal antibody that binds to heavy and light chains of mouse IgG. It's specifically designed for use in ELISA's and works very well in ELISAs as a secondary detection reagent

Q. Have you used ELISA before? Do you think ELISA reader is OK?
A. I've developed and validated lots of ELISA's from scratch and have also successfully developed and validated two multiplex ELISAs.
The plate reader is working fine as we are running other ELISA's at the moment too without problems.

Q. Was there no reading at all with serum and c-peptide?
A. We tested the detection only with the antigen (C-peptide and serum) immobilised onto the plate
The results for our diluent with skimmed milk as the blocker as are follows: The signal we got for the highest point on the standard curve was 63, the blank was 53 and for serum sample a: 55 and serum sample b: 53
The results with PBS with BSA as the blocker: The signal we got for the highest point on the standard curve was 62, the blank was 122 and for serum sample a: 67 and serum sample b: 61

When we tested the Capture Ab with the anti-species antibody we got similar results too:
For example, the signal we got for the highest point on the standard curve was 83, the blank was 50 and for serum sample a: 47 and serum sample b: 61

Many thanks,

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Answer

Thank you very much for confirming details.
The protein ab93903 has not been tested in ELISA and in combination with ab8297, ab8298 and ab48303 so we unfortunately do not hold any ELISA data for this product. This product was in fact used as blocking peptide with antibody ab14181. I have also contacted our laboratory regarding this and now waiting a reply from them. I will share their experience with you soon.
The further question I would like to ask is; were the concentration/ dilution of peptide, optimized in combination with antibodies?
Thank you very much for your cooperation.

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