Human C Reactive Protein ELISA Kit (CRP) (ab99995)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 34.29 pg/ml - 25000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human C Reactive Protein ELISA Kit (CRP)
See all C Reactive Protein kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
34.29 pg/ml - 25000 pg/ml -
Recovery
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 106.2 95% - 116% Serum 116.7 106% - 127% Plasma 102.2 93% - 112% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s C Reactive Protein (CRP) Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CRP in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 2 x 15ml CRP Microplate (12 x 8 well strips) 1 unit Biotinylated anti-Human CRP 2 vials Recombinant Human CRP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab99995 has been referenced in 25 publications.
- Jiang S et al. Oxidation of protein disulfide bonds by singlet oxygen gives rise to glutathionylated proteins. Redox Biol 38:101822 (2021). PubMed: 33338920
- Mateos J et al. Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection. Sci Rep 10:3844 (2020). PubMed: 32123229
- Ashiba H et al. Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor. Sensors (Basel) 20:N/A (2020). PubMed: 32512921
- Feng J et al. Clinical Significance of microRNA-146a in Patients with Ulcerative Colitis. Ann Clin Lab Sci 50:463-467 (2020). PubMed: 32826242
- Li C et al. Effect of Renal Denervation on Cardiac Function and Inflammatory Factors in Heart Failure After Myocardial Infarction. J Cardiovasc Pharmacol 76:602-609 (2020). PubMed: 32868626