Overview

  • Product name
    Human C Reactive Protein ELISA Kit (CRP)
    See all C Reactive Protein kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2.47 pg/ml - 600 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.2 95% - 116%
    Serum 116.7 106% - 127%
    Plasma 102.2 93% - 112%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s C Reactive Protein (CRP) Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CRP in serum, plasma, cell culture supernatants.


    This assay employs an antibody specific for Human CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.


     


     

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Sandwich ELISA- hu CRP (ab99995)
    Sandwich ELISA- hu CRP (ab99995)

    huCRP measured in biological fluids showing quantity (pg) per mL of sample tested.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab99995.

Protocols

References

This product has been referenced in:
  • Niu R  et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . Read more (PubMed: 28122020) »
  • Wan B  et al. Rhubarb vs. glycerin enema for treatment of critically ill patients with intra-abdominal hypertension. Exp Ther Med 14:855-861 (2017). Read more (PubMed: 28673010) »

See all 6 Publications for this product

Publishing research using ab99995? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

15 reviews or Q&A

10
Votes: Highest First


What is the shelf life of your assay kits?


The shelf life of each of our kits is indicated in the individual protocol booklet.

ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80&d...

Read More

For all those tests that I explained in previous email, I incubate them over night at 4C.
and dilution for HRP was once 300 fold (as described in the manual) and also when I ran the test
in parallel with HRP form albumin kit, I diluted 200 fol...

Read More

The lab sent the following comments and questions. Please let me know if you have the answers to those. I think the lab is trying to understand where exactly the problem might lie.

But I still think the standard curve should look better than w...

Read More

For all those tests that I explained in previous email, I incubate them over night at 4C.
and dilution for HRP was once 300 fold (as described in the manual) and also when I ran the test
in parallel with HRP form albumin kit, I diluted 200 fol...

Read More

Thank you so much for the additional information. I'll pass it on to the lab.

Should they have more suggestions, I will let you know.

I hope the 2 new kits will work as expected.

Please let me know if you have further questions.

I ran few more tests again based on what your lab recommended.

1- I used another kit with standards and my samples without dilution :
Result: same situation as previous kit for STD and still no detection for my samples.
my samples are ...

Read More

Thank you for your emails.

I apologize that I could not get back to you earlier, but our office was closed over the holidays.

I am very sorry to hear that your effort and attempts to try to get the kits to work were unsuccessful. I wi...

Read More

I confirm our PO 2527 of 11/09/2012.
So please send us new vial of RP-streptavidin concentrate item.

Thanks in advance and kind regards

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

I was just wondering if there is any progress in your investigation or not yet.
I appreciate if you please let me know as soon as you can, since I need to test my samples
as soon as it's possible.

Thank you for your patience.
I have heard back from the lab today with the following response:

"I am not quite sure what exactly the issue is with the standard curve. I plotted the values in a linear regression model and curve looks f...

Read More

antibody vials seem to be empty
lot GR91493-2 and lot GR95066-1

Thank you for your phone message.

I have emailed the lab yesterday but have not yet heard back. I will follow up with them today.

As for the antibody vial:

Item F detection antibody comes as a very concentrated, small volume ...

Read More

Dear Colleagues,
a customer of ours bought your AB99995 lot GR91493-2 (our PO 2527), but she found less HRP-streptavidin concentrate (Item G) than expected.
In fact she found only 40ul instead of 200ul (300X).
Can you please send us a ne...

Read More

Thank you for your message. I am sorry to hear there was a shortage of the HRP-streptavidin concentrate item in the ab99995 kit.

If this has been purchased in the last 6 months, I will be pleased to provide a replacement of this component. I...

Read More

a customer of ours has purchased your kit ab99995 (lot GR79069-3) but after dilution of buffer B, this buffer appears to be "opalescent"!.
Is this normal? Does the customer can use it? Will the kit work?

Please note that the cust...

Read More

Thank you for contacting us.

This is very normal, the buffer contains proteins which gets precipitated at low temperature. Please keep the buffer at room temperature for few minutes and then vortex it. The white opaque residue will go away. Read More

The two most recent CRP ELISA kits we ordered gave no signal at all with urine samples or the standards in the kit. An earlier kit from a different lot worked as expected.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up