Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 16 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman C Reactive Protein ELISA Kit (PTX1)
See all C Reactive Protein kits
Intra-assay Sample n Mean SD CV% Overall 4% Inter-assay Sample n Mean SD CV% Overall 11.3%
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
Assay typeSandwich (quantitative)
Sensitivity= 0.2 ng/ml
Range0.25 ng/ml - 16 ng/ml
Assay time4h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s C Reactive Protein (PTX1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein concentrations in Human plasma, serum, saliva, milk, urine and cell culture supernatants.
A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.
Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human C Reactive Protein Antibody 1 x 120µl C Reactive Protein Microplate (12 x 8 well strips) 1 unit C Reactive Protein Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml
FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Tissue specificityFound in plasma.
Sequence similaritiesBelongs to the pentaxin family.
Contains 1 pentaxin domain.
- Information by UniProt
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
ab108826 has been referenced in 1 publication.
- Strøm Halden TA et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). PubMed: 24452849