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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins
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RecombinantSimpleStep

Human Calbindin ELISA Kit (ab218270)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human Calbindin ELISA Kit (ab218270)
  • Human Calbindin standard curve comparison data
  • Example of human calbindin standard curve in Sample Diluent NS
  • Example of human calbindin standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of native calbindin in human urine
  • Interpolated concentrations of native calbindin in human brain tissue extract based on a 1,000 µg/mL extract load.
  • Sandwich ELISA - Human Calbindin ELISA Kit (ab218270)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.7 pg/ml
  • Range: 9.4 pg/ml - 600 pg/ml
  • Sample type: Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human Calbindin protein (ab82631)

View more associated products

Overview

  • Product name

    Human Calbindin ELISA Kit
    See all Calbindin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pooled urine 8 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Pooled urine 3 9.6%
  • Sample type

    Urine, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.7 pg/ml
  • Range

    9.4 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 115 114% - 117%
    Tissue Extracts 101 99% - 105%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Calbindin ELISA Kit (ab218270) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Calbindin protein in tissue extracts and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Calbindin with 3.7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Calbindin is a 260 amino acid protein encoded by the gene CALB1. Also, referred to as Calbindin D28, D-28K, and Vitamin D-dependent calcium-binding protein, CALB1 belongs to a family of calcium binding proteins which includes parvalbumin, calmodulin, calretinin S100G, and troponin C. CALB1 contains five EF-hand domains, and four calcium binding sites, although two sites have lost binding capacity for calcium. CALB1 functions in the absorption of calcium in the kidney and intestine, and in neural tissue CALB1 is involved in neural function by modulating calcium channel activity. Expressed in the distal tubule and in the proximal part of the collecting kidney ducts, CALB1 is thought to be a kidney injury marker.  

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Calbindin Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    20X Human Calbindin Detector Antibody 1 x 300µl
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BC 1 x 6ml
    Human Calbindin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
  • Function

    Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
  • Sequence similarities

    Belongs to the calbindin family.
    Contains 5 EF-hand domains.
  • Domain

    This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
  • Target information above from: UniProt accession P05937 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • avian-type
    • CAB27
    • CALB
    • CALB 1
    • CALB1
    • CALB1_HUMAN
    • Calbindin
    • Calbindin 1 28kDa
    • Calbindin D28
    • D 28K
    • D-28K
    • D28K
    • OTTHUMP00000166027
    • OTTHUMP00000225441
    • RTVL H protein
    • Vitamin D dependent calcium binding protein
    • Vitamin D dependent calcium binding protein avian type
    • Vitamin D-dependent calcium-binding protein
    see all
  • Database links

    • Entrez Gene: 793 Human
    • Omim: 114050 Human
    • SwissProt: P05937 Human
    • Unigene: 65425 Human

    Associated products

    • ELISA kits

      • Human Calbindin Matched Antibody Pair Kit (ab220139)

    Images

    • Other - Human Calbindin ELISA Kit (ab218270)
      Other - Human Calbindin ELISA Kit (ab218270)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human Calbindin standard curve comparison data
      Human Calbindin standard curve comparison data

      Standard curve comparison between human Calbindin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold sensitivity.

    • Example of human calbindin standard curve in Sample Diluent NS
      Example of human calbindin standard curve in Sample Diluent NS

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of human calbindin standard curve in 1X Cell Extraction Buffer PTR
      Example of human calbindin standard curve in 1X Cell Extraction Buffer PTR

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native calbindin in human urine
      Interpolated concentrations of native calbindin in human urine

      The concentrations of calbindin were measured in duplicate, interpolated from the calbindin standard curve and corrected for sample dilution. Undiluted sample was at 10%. The interpolated dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean calbindin concentration was determined to be 3.62 ng/mL in urine.

    • Interpolated concentrations of native calbindin in human brain tissue extract based on a 1,000 µg/mL extract load.
      Interpolated concentrations of native calbindin in human brain tissue extract based on a 1,000 µg/mL extract load.

      The concentrations of calbindin were measured in duplicate, interpolated from the calbindin standard curve, and corrected for sample dilution. The interpolated dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean calbindin concentration was determined to be 733 ng/mL of human brain tissue.

    • Sandwich ELISA - Human Calbindin ELISA Kit (ab218270)
      Sandwich ELISA - Human Calbindin ELISA Kit (ab218270)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab218270? Please let us know so that we can cite the reference in this datasheet.

    ab218270 has not yet been referenced specifically in any publications.

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