Overview

  • Product name

    Human Calbindin ELISA Kit
    See all Calbindin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pooled urine 8 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Pooled urine 3 9.6%
  • Sample type

    Urine, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.7 pg/ml
  • Range

    9.4 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 115 114% - 117%
    Tissue Extracts 101 99% - 105%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Calbindin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Calbindin protein in human tissue extracts and urine.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:



    • Samples in 1X Cell Extraction Buffer PTR: 3.7 pg/mL

    • Samples in Sample Diluent NS: 9.1 pg/mL

  • Notes

    Calbindin is a 260 amino acid protein encoded by the gene CALB1. Also, referred to as Calbindin D28, D-28K, and Vitamin D-dependent calcium-binding protein, CALB1 belongs to a family of calcium binding proteins which includes parvalbumin, calmodulin, calretinin S100G, and troponin C. CALB1 contains five EF-hand domains, and four calcium binding sites, although two sites have lost binding capacity for calcium. CALB1 functions in the absorption of calcium in the kidney and intestine, and in neural tissue CALB1 is involved in neural function by modulating calcium channel activity. Expressed in the distal tubule and in the proximal part of the collecting kidney ducts, CALB1 is thought to be a kidney injury marker.  

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Calbindin Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    20X Human Calbindin Detector Antibody 1 x 300µl
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BC 1 x 6ml
    Human Calbindin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
  • Sequence similarities

    Belongs to the calbindin family.
    Contains 5 EF-hand domains.
  • Domain

    This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
  • Information by UniProt
  • Alternative names

    • avian-type
    • CAB27
    • CALB
    • CALB 1
    • CALB1
    • CALB1_HUMAN
    • Calbindin
    • Calbindin 1 28kDa
    • Calbindin D28
    • D 28K
    • D-28K
    • D28K
    • OTTHUMP00000166027
    • OTTHUMP00000225441
    • RTVL H protein
    • Vitamin D dependent calcium binding protein
    • Vitamin D dependent calcium binding protein avian type
    • Vitamin D-dependent calcium-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab218270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human Calbindin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of calbindin were measured in duplicate, interpolated from the calbindin standard curve and corrected for sample dilution. Undiluted sample was at 10%. The interpolated dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean calbindin concentration was determined to be 3.62 ng/mL in urine.

  • The concentrations of calbindin were measured in duplicate, interpolated from the calbindin standard curve, and corrected for sample dilution. The interpolated dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean calbindin concentration was determined to be 733 ng/mL of human brain tissue.

Protocols

References

ab218270 has not yet been referenced specifically in any publications.

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