SimpleStep

Human CAMK2B ELISA Kit (ab234572)

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Overview

  • Product name

    Human CAMK2B ELISA Kit
    See all CaMKII beta kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 5 2.2%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 8.8%

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.28 ng/ml

  • Range

    3.13 ng/ml - 200 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 98 98% - 99%
    Tissue Extracts 106 102% - 109%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    CAMK2B in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CAMK2B protein in human cell and tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    CAMK2B (CaM kinase II subunit beta) is the beta chain of a widely expressed calcium/calmodulin-dependent kinase which is activated by calcium/calmodulin. CAMK2B is involved in spine and synapse formations, neuronal plasticity, and regulation of sarcoplasmic reticulum Ca2+ transport. ab234572 employs capture and detector antibodies raised against a fragment that is common to all eight isoforms of CAMK2B, and calibrated against the full length active protein of CAMK2B Isoform 2.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab234572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Other - Human CAMK2B ELISA Kit (ab234572)
    Other - Human CAMK2B ELISA Kit (ab234572)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human CAMK2B standard curve in 1X Cell Extraction Buffer PTR.
    Example of human CAMK2B standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CAMK2B in human SH-SY5Y cell extract, HEK293T cell extract, MCF-7 cell extract, A431 cell extract and A20 cell extract, based on a 500 µg/mL extract load.
    Interpolated concentrations of native CAMK2B in human SH-SY5Y cell extract, HEK293T cell extract, MCF-7 cell extract, A431 cell extract and A20 cell extract, based on a 500 µg/mL extract load.

    The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 200 ng/mL in SH-SY5Y cell extract, 64 ng/mL in HEK293T cell extract, 52 ng/mL in MCF-7 cell extract, 76 ng/mL in A431 cell extract and 13 ng/mL in A20 cell extract.

  • Interpolated concentrations of native CAMK2B in human brain tissue extract, based on a 100 µg/mL extract load.
    Interpolated concentrations of native CAMK2B in human brain tissue extract, based on a 100 µg/mL extract load.

    The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 37 ng/mL in human brain tissue extract.

  • Interpolated concentrations of native CAMK2B in mouse RAW 264.7 cell extract and rat PC-12 cell extract based on a 500 µg/mL extract load.
    Interpolated concentrations of native CAMK2B in mouse RAW 264.7 cell extract and rat PC-12 cell extract based on a 500 µg/mL extract load.

    The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 73 ng/mL in mouse RAW 264.7 cell extract and 195 ng/mL in rat PC-12 cell extract.

Protocols

References

ab234572 has not yet been referenced specifically in any publications.

Publishing research using ab234572? Please let us know so that we can cite the reference in this datasheet.

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