RecombinantSimpleStep

Human CAMK2B ELISA Kit (ab234572)

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Other - Human CAMK2B ELISA Kit (ab234572)
  • Example of human CAMK2B standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native CAMK2B in human SH-SY5Y cell extract, HEK293T cell extract, MCF-7 cell extract, A431 cell extract and A20 cell extract, based on a 500 µg/mL extract load.
  • Interpolated concentrations of native CAMK2B in human brain tissue extract, based on a 100 µg/mL extract load.
  • Interpolated concentrations of native CAMK2B in mouse RAW 264.7 cell extract and rat PC-12 cell extract based on a 500 µg/mL extract load.
  • Sandwich ELISA - Human CAMK2B ELISA Kit (ab234572)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.28 ng/ml
  • Range: 3.13 ng/ml - 200 ng/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CAMK2B ELISA Kit
    See all CaMKII beta kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 5 2.2%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 8.8%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.28 ng/ml

  • Range

    3.13 ng/ml - 200 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 98 98% - 99%
    Tissue Extracts 106 102% - 109%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CAMK2B ELISA Kit (ab234572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CAMK2B protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CAMK2B with 1.28 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Images

  • Other - Human CAMK2B ELISA Kit (ab234572)
    Other - Human CAMK2B ELISA Kit (ab234572)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human CAMK2B standard curve in 1X Cell Extraction Buffer PTR.
    Example of human CAMK2B standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CAMK2B in human SH-SY5Y cell extract, HEK293T cell extract, MCF-7 cell extract, A431 cell extract and A20 cell extract, based on a 500 µg/mL extract load.
    Interpolated concentrations of native CAMK2B in human SH-SY5Y cell extract, HEK293T cell extract, MCF-7 cell extract, A431 cell extract and A20 cell extract, based on a 500 µg/mL extract load.

    The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 200 ng/mL in SH-SY5Y cell extract, 64 ng/mL in HEK293T cell extract, 52 ng/mL in MCF-7 cell extract, 76 ng/mL in A431 cell extract and 13 ng/mL in A20 cell extract.

  • Interpolated concentrations of native CAMK2B in human brain tissue extract, based on a 100 µg/mL extract load.
    Interpolated concentrations of native CAMK2B in human brain tissue extract, based on a 100 µg/mL extract load.

    The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 37 ng/mL in human brain tissue extract.

  • Interpolated concentrations of native CAMK2B in mouse RAW 264.7 cell extract and rat PC-12 cell extract based on a 500 µg/mL extract load.
    Interpolated concentrations of native CAMK2B in mouse RAW 264.7 cell extract and rat PC-12 cell extract based on a 500 µg/mL extract load.

    The concentrations of CAMK2B were measured in duplicate and interpolated from the CAMK2B standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CAMK2B concentration was determined to be 73 ng/mL in mouse RAW 264.7 cell extract and 195 ng/mL in rat PC-12 cell extract.

  • Sandwich ELISA - Human CAMK2B ELISA Kit (ab234572)
    Sandwich ELISA - Human CAMK2B ELISA Kit (ab234572)
    To learn more about the advantages of recombinant antibodies see here.

References (1)

Publishing research using ab234572? Please let us know so that we can cite the reference in this datasheet.

ab234572 has been referenced in 1 publication.

  • Jayabalan N  et al. Quantitative Proteomics by SWATH-MS Suggest an Association Between Circulating Exosomes and Maternal Metabolic Changes in Gestational Diabetes Mellitus. Proteomics 19:e1800164 (2019). PubMed: 30536821

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