Overview

  • Product name
    Human Cancer Antigen CA15-3 ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 5 U/ml
  • Range
    5 U/ml - 240 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108633 Cancer Antigen CA15-3 Human ELISA Kit is intended for the quantitative determination of the Cancer Antigen CA15-3 concentration in human serum.

    Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today, with approximately 180,000 new cases diagnosed every year.

    There are a number of tumor markers that can help clinicians to identify and diagnose which breast cancer patients will have aggressive disease and which will have an indolent course. These markers include estrogen and progesterone receptors, DNA ploidy and percent-S phase profile, epidermal growth factor receptor, HER-2/neu oncogene, p53 tumor suppressor gene, cathepsin D, proliferation markers and CA15-3. CA15-3 is most useful for monitoring patients post-operatively for recurrence, particularly metastatic diseases. 96% of patients with local and systemic recurrence have elevated CA15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3 is associated with progression of carcinoma. A 50% decrease in serum CA15-3 is associated with response to treatment. CA15-3 is more sensitive than CEA in early detection of breast cancer recurrence.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

Protocols

References

ab108633 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

We recieved an answer from the collaborating lab regarding your question:

"We purchased at least 100 CA15-3 patient samples from a vendor(s). The vendor provided us the CA15-3 value for each sample tested by Abbott's CA15-3 test. We then tested these samples using our CA15-3 kits and made sure the values obtained using our kits were similar to/correlated with the values (from Abbott's kits) provided by the vendor.

We don't know exactly how much is 1 Unit. There is no universal standard but everyone knows that the cut-off for CA15-3 is 35 U/ml. "

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Question
Answer

Unfortunately, we have not tested this kit with recombinant CA15-3 and therefore have no information as to whether it will work with a recombinant protein. This also means that this product would not be covered by our abpromise guarantee if used with recombinant CA15-3.

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Answer

Thank you for your inquiry.

I am sorry to confirm that we do not have any ELISA kits to detect anti-HER2 and anti-CA15-3 antibodies.

If I understand correctly, you would like to detect the actual antibodies from serum etc? There is the possibility of establishing an ELISA manually:

Coat plates with protein (CA15-3 Human), block, add serum samples of interest (antibodies reacting with CA15-3 Human will bind wash away other antibodies, detect anti-CA15-3 antibodies with secondary anti human IgG + IgM conjugated to enzyme.

I am sorry I did not have a positive answer to your question on this occasion and hope that this information is nevertheless helpful.

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Question
Answer

Thank you for your patience.

This is the additional information I received from the supplying lab:

We purchased at least 100 CA15-3 patient samples from a vendor. The vendor provided us the CA15-3 value for each sample tested by Abbott's CA15-3 test. We then tested these samples using our CA15-3 kit and made sure the values obtained using our kit were similar to/correlated with the values (from Abbott's test) provided by the vendor.

We don't know exactly how much is 1 Unit. There is no universal standard but it isknown that the cut-off for CA15-3 is 35 U/ml.

I hope this additional information is of some more help to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us.

I have received this information from the lab so far:
There is no WHO standard or international standard for this product. We calibrate the CA15-3 kit against the Abbott's test.

I have inquired about more details for the Abbott's test and let you know what I hear back.
Thank you for your patience.

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Answer

Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/ml.  U is an arbitrary assigned number.  From then on, most (if not all) other companies manufacturing kits for ELISA detection of the cancer antigens calibrated their standards against the original kit.   The standards used in the kit are still calibrated this way. 2, I am sorry it would not be possible to convert from U to ng. I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.      

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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