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Human Cancer Antigen CA15-3 ELISA Kit (ab108633)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (6)References (1)

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- Cancer Antigen CA15-3 Human ELISA Kit (ab108633)

    Key features and details

    • Sensitivity: 5 U/ml
    • Range: 5 U/ml - 240 U/ml
    • Sample type: Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Cancer Antigen CA15-3 ELISA Kit
    • Detection method

      Colorimetric
    • Sample type

      Serum
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      = 5 U/ml
    • Range

      5 U/ml - 240 U/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      ab108633 Cancer Antigen CA15-3 Human ELISA Kit is intended for the quantitative determination of the Cancer Antigen CA15-3 concentration in human serum.

      Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today, with approximately 180,000 new cases diagnosed every year.

      There are a number of tumor markers that can help clinicians to identify and diagnose which breast cancer patients will have aggressive disease and which will have an indolent course. These markers include estrogen and progesterone receptors, DNA ploidy and percent-S phase profile, epidermal growth factor receptor, HER-2/neu oncogene, p53 tumor suppressor gene, cathepsin D, proliferation markers and CA15-3. CA15-3 is most useful for monitoring patients post-operatively for recurrence, particularly metastatic diseases. 96% of patients with local and systemic recurrence have elevated CA15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3 is associated with progression of carcinoma. A 50% decrease in serum CA15-3 is associated with response to treatment. CA15-3 is more sensitive than CEA in early detection of breast cancer recurrence.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Washing Solution 1 x 50ml
      CA15-3 Standard 1 - 0 U/mL  1 unit
      CA15-3 Standard 2 - 15 U/mL 1 unit
      CA15-3 Standard 3 - 30 U/mL  1 unit
      CA15-3 Standard 4 - 60 U/mL 1 unit
      CA15-3 Standard 5 - 120 U/mL 1 unit
      CA15-3 Standard 6 - 240 U/mL  1 unit
      Enzyme Conjugate Concentrate 1 x 1ml
      Enzyme Conjugate Diluent 1 x 21ml
      IgG Sample Diluent 1 x 100ml
      Mouse Monoclonal anti-CA15-3 coated microtiter plate 1 unit
      Stop Solution 1 x 11ml
      TMB Reagent 1 x 11ml
    • Research areas

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      • Kits
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      • Cancer
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      • Biomarker Panels

    Associated products

      Images

      • - Cancer Antigen CA15-3 Human ELISA Kit (ab108633)
        - Cancer Antigen CA15-3 Human ELISA Kit (ab108633)

        Representative Standard Curve using ab108633. 

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab108633? Please let us know so that we can cite the reference in this datasheet.

      ab108633 has been referenced in 1 publication.

      • Dowling P  et al. Transferrin-bound proteins as potential biomarkers for advanced breast cancer patients. BBA Clin 2:24-30 (2014). PubMed: 26673961

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Question



      Regarding the CA15-3 elisa kit: the standard solutions are in U/ml. MUC1 protein that you provide is mg/ml. In case we use your protein for an immunoassay and your kit as a reference method is there a possibility to convert mg/ml in U/ml? do you have a conversion factor? or so you know the threshold value of MUC1 for cancer in mg/ml?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 25 2013

      Answer

      We recieved an answer from the collaborating lab regarding your question:

      "We purchased at least 100 CA15-3 patient samples from a vendor(s). The vendor provided us the CA15-3 value for each sample tested by Abbott's CA15-3 test. We then tested these samples using our CA15-3 kits and made sure the values obtained using our kits were similar to/correlated with the values (from Abbott's kits) provided by the vendor.

      We don't know exactly how much is 1 Unit. There is no universal standard but everyone knows that the cut-off for CA15-3 is 35 U/ml. "

      Read More

      Abcam Scientific Support

      Answered on Jan 25 2013

      Question

      Can the kit be used to detect recombinant CA15-3?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 15 2013

      Answer

      Unfortunately, we have not tested this kit with recombinant CA15-3 and therefore have no information as to whether it will work with a recombinant protein. This also means that this product would not be covered by our abpromise guarantee if used with recombinant CA15-3.

      Read More

      Abcam Scientific Support

      Answered on Jan 15 2013

      Question

      Inquiry: Hi I'm student from MOROCCO I've already asked you about Elisa kits for HER2 AND CA15-3 proteins detection our supplier told us that he already order those kits from your laboratory and now we need Elisa kits for detection of antibodies anti-HER2 and anti-CA15-3 I didn't find this in your list products and i'm wondering if you have this 2 kits we need 5 plates for each kit thanks bests regards

      Read More

      Abcam community

      Verified customer

      Asked on Dec 17 2012

      Answer

      Thank you for your inquiry.

      I am sorry to confirm that we do not have any ELISA kits to detect anti-HER2 and anti-CA15-3 antibodies.

      If I understand correctly, you would like to detect the actual antibodies from serum etc? There is the possibility of establishing an ELISA manually:

      Coat plates with protein (CA15-3 Human), block, add serum samples of interest (antibodies reacting with CA15-3 Human will bind wash away other antibodies, detect anti-CA15-3 antibodies with secondary anti human IgG + IgM conjugated to enzyme.

      I am sorry I did not have a positive answer to your question on this occasion and hope that this information is nevertheless helpful.

      Read More

      Abcam Scientific Support

      Answered on Dec 17 2012

      Question

      what does 1 U/ml of the standard corresponds to in mg/ml?

      Read More

      Abcam community

      Verified customer

      Asked on May 29 2012

      Answer

      Thank you for your patience.

      This is the additional information I received from the supplying lab:

      We purchased at least 100 CA15-3 patient samples from a vendor. The vendor provided us the CA15-3 value for each sample tested by Abbott's CA15-3 test. We then tested these samples using our CA15-3 kit and made sure the values obtained using our kit were similar to/correlated with the values (from Abbott's test) provided by the vendor.

      We don't know exactly how much is 1 Unit. There is no universal standard but it isknown that the cut-off for CA15-3 is 35 U/ml.

      I hope this additional information is of some more help to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 29 2012

      Question

      what does 1 U/ml of the standard corresponds to in mg/ml?

      Read More

      Abcam community

      Verified customer

      Asked on May 23 2012

      Answer

      Thank you for contacting us.

      I have received this information from the lab so far:
      There is no WHO standard or international standard for this product. We calibrate the CA15-3 kit against the Abbott's test.

      I have inquired about more details for the Abbott's test and let you know what I hear back.
      Thank you for your patience.

      Read More

      Abcam Scientific Support

      Answered on May 23 2012

      Question

       Dear technical team,   Our customer would like to purchase these kits and wants to know the meaning of "unit"  in Sensitivity : 5U/ml.   1. unit definition   2. is it possible conversion of unit  to  ng?   Check this please.     Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 18 2011

      Answer

      Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/ml.  U is an arbitrary assigned number.  From then on, most (if not all) other companies manufacturing kits for ELISA detection of the cancer antigens calibrated their standards against the original kit.   The standards used in the kit are still calibrated this way. 2, I am sorry it would not be possible to convert from U to ng. I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.      

      Read More

      Abcam Scientific Support

      Answered on Oct 18 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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