Human Cancer Antigen CA15-3 ELISA Kit (ab108633)

We recieved an answer from the collaborating lab regarding your question:

"We purchased at least 100 CA15-3 patient samples from a vendor(s). The vendor provided us the CA15-3 value for each sample tested by Abbott's CA15-3 test. We then tested these samples using our CA15-3 kits and made sure the values obtained using our kits were similar to/correlated with the values (from Abbott's kits) provided by the vendor.

We don't know exactly how much is 1 Unit. There is no universal standard but everyone knows that the cut-off for CA15-3 is 35 U/ml. "

Unfortunately, we have not tested this kit with recombinant CA15-3 and therefore have no information as to whether it will work with a recombinant protein. This also means that this product would not be covered by our abpromise guarantee if used with recombinant CA15-3.

Thank you for your inquiry.

I am sorry to confirm that we do not have any ELISA kits to detect anti-HER2 and anti-CA15-3 antibodies.

If I understand correctly, you would like to detect the actual antibodies from serum etc? There is the possibility of establishing an ELISA manually:

Coat plates with protein (CA15-3 Human), block, add serum samples of interest (antibodies reacting with CA15-3 Human will bind wash away other antibodies, detect anti-CA15-3 antibodies with secondary anti human IgG + IgM conjugated to enzyme.

I am sorry I did not have a positive answer to your question on this occasion and hope that this information is nevertheless helpful.

Thank you for your patience.

This is the additional information I received from the supplying lab:

We purchased at least 100 CA15-3 patient samples from a vendor. The vendor provided us the CA15-3 value for each sample tested by Abbott's CA15-3 test. We then tested these samples using our CA15-3 kit and made sure the values obtained using our kit were similar to/correlated with the values (from Abbott's test) provided by the vendor.

We don't know exactly how much is 1 Unit. There is no universal standard but it isknown that the cut-off for CA15-3 is 35 U/ml.

I hope this additional information is of some more help to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

I have received this information from the lab so far:
There is no WHO standard or international standard for this product. We calibrate the CA15-3 kit against the Abbott's test.

I have inquired about more details for the Abbott's test and let you know what I hear back.
Thank you for your patience.

Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/ml.  U is an arbitrary assigned number.  From then on, most (if not all) other companies manufacturing kits for ELISA detection of the cancer antigens calibrated their standards against the original kit.   The standards used in the kit are still calibrated this way. 2, I am sorry it would not be possible to convert from U to ng. I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.