Human Cancer Antigen CA19-9 ELISA Kit (ab108642)

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I am sorry I could not find a document where it say the kit is tested with plasma samples - this might not have been tried in past. I would recommend using this kit as we discussed on phone; I don't see a reason why this kit should not work with plasma samples. Please let me know if you experience any problem.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for your enquiry.

I can confirm that The CA19-9 standards in the ab108642 kit are prepared from partially purified CA19-9 antigen. The source is human fluids, not a recombinant protein. The standards are calibrated against other kits.

I hope this information is helpful. If you have any further questions, please do not hesitate to contact us.

Thank you for your enquriy.

We sincerely apologize that there has been an error on the datasheet information. The Enzyme Conjugate Concentrate (12X) component volume is 1.1ml, not 2ml as previously stated. Please rest assured that the volume is more than sufficient for your experiment and we regret that the error was not corrected earlier.

Thank you for your message and inquiry.

I can confirm that in this assay (ab108642 - Cancer Antigen CA19-9 Human ELISA Kit) the same antibody is used for both capture and detection. The subclass of this antibody is IgG1.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for letting us know. I am very sorry to hear that you have been experiencing difficulties with this products (ab108642: Cancer Antigen CA19-9 Human ELISA Kit).

We have been using Corning's micotiter plates for more than 15 years. This is the first time we heard that the strips fall off from the frame. If we hear more of the same problem happens to our other customers, we will do some investigation.

The second antibody is a mouse monoclonal.

If you need any further assistance, please do not hesitate to contact me again.

Thank you for your inquiry. I am happy to confirm that this kit is normally used with neat undiluted samples. The standard curve ranges from 0 - 600 U/mL with a estimated minimum detectable concentration of 10 U/mL. This kit is for research use only, but healthy men and women are expected to have CA19-9 levels below 35 U/mL. Some cancerous/disease state samples will have levels outside the upper limit of detection. When testing the kit in house, we normally perform a 1:10 and 1:20 dilution for re-testing the out of range samples. Serum samples demonstrating gross lipemia, gross hemolysis,or turbidity should not be used with this test. We offer bulk discounts from 5 items. I send you a quotation for four items as requested. When you decide to order please contact our customer service team(mailto:orders@abcam.com). I hope this information is helpful and wish you a good start into the new year.

Thank you very much for your email. It seems that wehave sent the updated version of the productwiththe non-updated version ofproductdatasheet and protocol.There have been few changes in the protocol that were not updated on the website. We sincerely apologize for the delays. Please find attached the latest version of the protocol that we should have sent with the product. The kits are absolutely fine to use with the attached protocol. I would suggest using the kits as normal and contacting us again if any of these kits fails. If however this error has already affected your experiment then pleases contact me again asap I will be happy to arrange credit notes or free of charge replacement kits. Once again I apologize for the error; please do contact us if you need further assistance. Best of luck with your reserach. Have a prosperous New Year 2012.

Sorry for the delay in getting back to you, I now have confirmation of the information requested. As we had assumed, the standards are labeled 1-6 from 0 to 600 U/mL, i.e standard 1 contains 0 U/ml, standard 2 contains 25 U/ml and so on with standard 6 containing 600 U/ml. I apologise for any confusion in this regard. Thank you for bringing this issue to our attention, we will be updating the datasheets accordingly. Please let me know if I can be of any further assistance.

Thank you for your inquiry. This kit has a shelf life of 1 year.  For the lots that you ordered, we would expect them to be good until October 2012 when stored properly. I hope this information helps.  Please contact us with any other questions.

Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/ml.  U is an arbitrary assigned number.  From then on, most (if not all) other companies manufacturing kits for ELISA detection of the cancer antigens calibrated their standards against the original kit.   The standards used in the kit are still calibrated this way. 2, I am sorry it would not be possible to convert from U to ng. I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.