For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    human-cancer-antigen-ca19-9-elisa-kit-ab108642.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cancer Tumor biomarkers Tumor antigens
Share by email

Human Cancer Antigen CA19-9 ELISA Kit (ab108642)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (14)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Cancer Antigen CA19-9 Human ELISA Kit (ab108642)

    Key features and details

    • Sensitivity: 5 U/ml
    • Range: 5 U/ml - 600 U/ml
    • Sample type: Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    You may also be interested in

    Primary
    Product image
    Anti-Flotillin 1 antibody [EPR6041] (ab133497)
    Primary
    Product image
    Anti-CD63 antibody [MX-49.129.5] - BSA and Azide free (ab213092)
    ELISA
    Product image
    Human Cytokeratin 18 ELISA Kit (ab227896)

    View more associated products

    Overview

    • Product name

      Human Cancer Antigen CA19-9 ELISA Kit
    • Detection method

      Colorimetric
    • Sample type

      Serum
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      = 5 U/ml
    • Range

      5 U/ml - 600 U/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      ab108642 Cancer Antigen CA19-9 Human ELISA Kit is intended for the quantitative determination of the Cancer Antigen CA19-9 concentration in Human serum.


      A group of mucin type glycoprotein Sialosyl Lewis Antigens (SLA), such as CA19-9 and CA19-5, have come to be recognized as circulating cancer associated antigens for gastrointestinal cancer. Cancer Antigen CA19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of Cancer Antigen CA19-9 in tissues is consistent with the quantitative determination of higher CA19-9 concentrations in cancer than in normal or inflamed tissues. Recent reports indicate that the serum CA19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Research studies demonstrate that serum CA19-9 values may have utility in monitoring subjects with the above-mentioned diagnosed malignancies. It has been shown that a persistent elevation in serum CA19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum Cancer Antigen CA19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining Cancer Antigen CA19-9 value may be indicative of a favorable prognosis and good response to treatment.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Washing Solution 1 x 50ml
      Assay Buffer 1 x 13ml
      Cancer Antigen CA19-9 Reference Standard 1, 0 U/ml 1 x 0.5ml
      Cancer Antigen CA19-9 Reference Standard 2, 25 U/ml 1 x 0.5ml
      Cancer Antigen CA19-9 Reference Standard 3, 75 U/ml 1 x 0.5ml
      Cancer Antigen CA19-9 Reference Standard 4, 150 U/ml 1 x 0.5ml
      Cancer Antigen CA19-9 Reference Standard 5, 300 U/ml 1 x 0.5ml
      Cancer Antigen CA19-9 Reference Standard 6, 600 U/ml 1 x 0.5ml
      Enzyme Conjugate Concentrate 1 x 1.1ml
      Enzyme Conjugate Diluent 1 x 13ml
      Murine monoclonal anti-Cancer Antigen CA19-9 coated microtiter plate. 1 unit
      Stop Solution 1 x 11ml
      TMB Reagent 1 x 11ml
    • Research areas

      • Cancer
      • Tumor biomarkers
      • Tumor antigens
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Tumor biomarkers ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cancer proteins ELISA kits
      • Cancer
      • Cancer Biomarker Panels
      • Biomarker Panels
    • Alternative names

      • Gastrointestinal Cancer Antigen CA19-9

    Images

    • ELISA - Cancer Antigen CA19-9 Human ELISA Kit (ab108642)
      ELISA - Cancer Antigen CA19-9 Human ELISA Kit (ab108642)
      Cancer Antigen CA19-9 Standard Curve

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab108642? Please let us know so that we can cite the reference in this datasheet.

    ab108642 has been referenced in 1 publication.

    • Melo SA  et al. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature 523:177-82 (2015). PubMed: 26106858

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 14 Abreviews or Q&A

    Question

    Can I use the kit ab108642 with plasma samples?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    I am sorry I could not find a document where it say the kit is tested with plasma samples - this might not have been tried in past. I would recommend using this kit as we discussed on phone; I don't see a reason why this kit should not work with plasma samples. Please let me know if you experience any problem.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Question

    Dear technical team,

    Our customer purchased ab108642 and she would like to know if standard is recombinant or endogenous protein.
    Please let me know if you can provide.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 30 2012

    Answer

    Thank you for your enquiry.

    I can confirm that The CA19-9 standards in the ab108642 kit are prepared from partially purified CA19-9 antigen. The source is human fluids, not a recombinant protein. The standards are calibrated against other kits.

    I hope this information is helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Jul 30 2012

    Question

    What is the volume of Enzyme Conjugate Concentrate (12X)?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2012

    Answer

    Thank you for your enquriy.

    We sincerely apologize that there has been an error on the datasheet information. The Enzyme Conjugate Concentrate (12X) component volume is 1.1ml, not 2ml as previously stated. Please rest assured that the volume is more than sufficient for your experiment and we regret that the error was not corrected earlier.

    Read More

    Abcam Scientific Support

    Answered on Jul 02 2012

    Question

    Thank you very much for your kind reply. Can you also
    provide me with information on the isotypes of the primary
    and secundary antibodies.
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 27 2012

    Answer

    Thank you for your message and inquiry.

    I can confirm that in this assay (ab108642 - Cancer Antigen CA19-9 Human ELISA Kit) the same antibody is used for both capture and detection. The subclass of this antibody is IgG1.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Mar 27 2012

    Question

    Dear Sirs,
    We regret to inform that some wells have released themselves from the plaque and almost compromised the outcome of the analysis. Fortunately these were in the zone containing the standards. I would also would like to know in which specie was the
    secondary antibody originated from. I have looked for this information in the protocol booklet but I don’t seem to find it.
    Yours sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Thank you for letting us know. I am very sorry to hear that you have been experiencing difficulties with this products (ab108642: Cancer Antigen CA19-9 Human ELISA Kit).

    We have been using Corning's micotiter plates for more than 15 years. This is the first time we heard that the strips fall off from the frame. If we hear more of the same problem happens to our other customers, we will do some investigation.

    The second antibody is a mouse monoclonal.

    If you need any further assistance, please do not hesitate to contact me again.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2012

    Question

    Hi there, I am looking into CA19.9 ELISA kits to determine CA19.9 conc. levels in our human serum samples. We have normal serum samples as well as cancer serum samples (pancreatic and colorectal cancer samples). Can you please quote me the price per kit? We will require 4 kits and were wondering if you also offered bulk discounts. The kit I am interested in is cat no. is ab108642. Also if you could suggest if human serum samples can be tested neat or have you got a preferred dilution that you use with your kit. I very much look forward to hear from you. Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 30 2011

    Answer

    Thank you for your inquiry. I am happy to confirm that this kit is normally used with neat undiluted samples. The standard curve ranges from 0 - 600 U/mL with a estimated minimum detectable concentration of 10 U/mL. This kit is for research use only, but healthy men and women are expected to have CA19-9 levels below 35 U/mL. Some cancerous/disease state samples will have levels outside the upper limit of detection. When testing the kit in house, we normally perform a 1:10 and 1:20 dilution for re-testing the out of range samples. Serum samples demonstrating gross lipemia, gross hemolysis,or turbidity should not be used with this test. We offer bulk discounts from 5 items. I send you a quotation for four items as requested. When you decide to order please contact our customer service team(mailto:orders@abcam.com). I hope this information is helpful and wish you a good start into the new year.

    Read More

    Abcam Scientific Support

    Answered on Dec 30 2011

    Question

    Our customer purchased19 ELISA kits from abcam at a time. He had purchase order 8 of ab108653, 11 of ab108642 and 10 of antibodies(ab52652). 5 kits of ab108642received last week and 6 kits arrived at Dawinbio today. All the kitshave short volume of CA19-9 conjugate Diluent. It mentioned 13ml on the website and product package also 13ml but follow the protocol, it should be 24ml and also needs this volume for 96well test. Actually the customer ordered 4 kits of ab108633 this Monday but after he found out all the kits have the same problem, hewas very upset and wants to cancel his order and doesn't want to use abcam. We persuaded him to use rest of products but about ab108642, he insists credit note for those 11kits. I have attached picture, red cap bottle is CA19-9 conjugate diluent (13ml) and all the kitshavesame size vial. Please arrange credit notes for him. He asked two things about this kit, 1. standard information ; is it recombinant or endogenous protein.. 2. the reasonfor short volume package. Please let me know. Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 29 2011

    Answer

    Thank you very much for your email. It seems that wehave sent the updated version of the productwiththe non-updated version ofproductdatasheet and protocol.There have been few changes in the protocol that were not updated on the website. We sincerely apologize for the delays. Please find attached the latest version of the protocol that we should have sent with the product. The kits are absolutely fine to use with the attached protocol. I would suggest using the kits as normal and contacting us again if any of these kits fails. If however this error has already affected your experiment then pleases contact me again asap I will be happy to arrange credit notes or free of charge replacement kits. Once again I apologize for the error; please do contact us if you need further assistance. Best of luck with your reserach. Have a prosperous New Year 2012.

    Read More

    Abcam Scientific Support

    Answered on Dec 29 2011

    Question

    Phone enquiry regarding to standard samples provided with the kit (labelled 1-6). Which contains which concentration of CA19-9 standard?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 21 2011

    Answer

    Sorry for the delay in getting back to you, I now have confirmation of the information requested. As we had assumed, the standards are labeled 1-6 from 0 to 600 U/mL, i.e standard 1 contains 0 U/ml, standard 2 contains 25 U/ml and so on with standard 6 containing 600 U/ml. I apologise for any confusion in this regard. Thank you for bringing this issue to our attention, we will be updating the datasheets accordingly. Please let me know if I can be of any further assistance.

    Read More

    Abcam Scientific Support

    Answered on Oct 21 2011

    Question

    Thank you for the CA19.9 ELISA kits (cat: ab108642) we have now safely received them. I just had one question, on these kits it doesn’t specify the expiration date of the kits or the reagents. Could you please send me the expiration date of these kits so that I can make a note of them on my lab study book.    

    Read More

    Abcam community

    Verified customer

    Asked on Oct 20 2011

    Answer

    Thank you for your inquiry. This kit has a shelf life of 1 year.  For the lots that you ordered, we would expect them to be good until October 2012 when stored properly. I hope this information helps.  Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 20 2011

    Question

     Dear technical team,   Our customer would like to purchase these kits and wants to know the meaning of "unit"  in Sensitivity : 5U/ml.   1. unit definition   2. is it possible conversion of unit  to  ng?   Check this please.     Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2011

    Answer

    Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/ml.  U is an arbitrary assigned number.  From then on, most (if not all) other companies manufacturing kits for ELISA detection of the cancer antigens calibrated their standards against the original kit.   The standards used in the kit are still calibrated this way. 2, I am sorry it would not be possible to convert from U to ng. I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.      

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2011

    1-10 of 14 Abreviews or Q&A

    •  Previous
    • 1
    • 2
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.