Human Cancer Antigen CA19-9 ELISA Kit (ab108642)

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Overview

  • Product name
    Human Cancer Antigen CA19-9 ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 5 U/ml
  • Range
    5 U/ml - 600 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108642 Cancer Antigen CA19-9 Human ELISA Kit is intended for the quantitative determination of the Cancer Antigen CA19-9 concentration in Human serum.


    A group of mucin type glycoprotein Sialosyl Lewis Antigens (SLA), such as CA19-9 and CA19-5, have come to be recognized as circulating cancer associated antigens for gastrointestinal cancer. Cancer Antigen CA19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of Cancer Antigen CA19-9 in tissues is consistent with the quantitative determination of higher CA19-9 concentrations in cancer than in normal or inflamed tissues. Recent reports indicate that the serum CA19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Research studies demonstrate that serum CA19-9 values may have utility in monitoring subjects with the above-mentioned diagnosed malignancies. It has been shown that a persistent elevation in serum CA19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum Cancer Antigen CA19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining Cancer Antigen CA19-9 value may be indicative of a favorable prognosis and good response to treatment.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Images

  • ELISA - Cancer Antigen CA19-9 Human ELISA Kit (ab108642)
    ELISA - Cancer Antigen CA19-9 Human ELISA Kit (ab108642)
    Cancer Antigen CA19-9 Standard Curve

Protocols

References

ab108642 has not yet been referenced specifically in any publications.

Publishing research using ab108642? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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14 reviews or Q&A

10
Votes: Highest First

Can I use the kit ab108642 with plasma samples?

Thank you for contacting us.

I am sorry I could not find a document where it say the kit is tested with plasma samples - this might not have been tried in past. I would recommend using this kit as we discussed on phone; I don't see a reason wh...

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Dear technical team,

Our customer purchased ab108642 and she would like to know if standard is recombinant or endogenous protein.
Please let me know if you can provide.

Best regards

Thank you for your enquiry.

I can confirm that The CA19-9 standards in the ab108642 kit are prepared from partially purified CA19-9 antigen. The source is human fluids, not a recombinant protein. The standards are calibrated against other ki...

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What is the volume of Enzyme Conjugate Concentrate (12X)?

Thank you for your enquriy.

We sincerely apologize that there has been an error on the datasheet information. The Enzyme Conjugate Concentrate (12X) component volume is 1.1ml, not 2ml as previously stated. Please rest assured that the volume...

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Thank you very much for your kind reply. Can you also
provide me with information on the isotypes of the primary
and secundary antibodies.
Best regards,

Thank you for your message and inquiry.

I can confirm that in this assay (ab108642 - Cancer Antigen CA19-9 Human ELISA Kit) the same antibody is used for both capture and detection. The subclass of this antibody is IgG1.

I hope this...

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Dear Sirs,
We regret to inform that some wells have released themselves from the plaque and almost compromised the outcome of the analysis. Fortunately these were in the zone containing the standards. I would also would like to know in which speci...

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Thank you for letting us know. I am very sorry to hear that you have been experiencing difficulties with this products (ab108642: Cancer Antigen CA19-9 Human ELISA Kit).

We have been using Corning's micotiter plates for more than 15 years. T...

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Hi there, I am looking into CA19.9 ELISA kits to determine CA19.9 conc. levels in our human serum samples. We have normal serum samples as well as cancer serum samples (pancreatic and colorectal cancer samples). Can you please quote me the price per k...

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Thank you for your inquiry. I am happy to confirm that this kit is normally used with neat undiluted samples. The standard curve ranges from 0 - 600 U/mL with a estimated minimum detectable concentration of 10 U/mL. This kit is for research use only, b...

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Our customer purchased19 ELISA kits from abcam at a time. He had purchase order 8 of ab108653, 11 of ab108642 and 10 of antibodies(ab52652). 5 kits of ab108642received last week and 6 kits arrived at Dawinbio today. All the kitshave short volume of CA1...

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Thank you very much for your email. It seems that wehave sent the updated version of the productwiththe non-updated version ofproductdatasheet and protocol.There have been few changes in the protocol that were not updated on the website. We sincerely a...

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Phone enquiry regarding to standard samples provided with the kit (labelled 1-6). Which contains which concentration of CA19-9 standard?

Sorry for the delay in getting back to you, I now have confirmation of the information requested. As we had assumed, the standards are labeled 1-6 from 0 to 600 U/mL, i.e standard 1 contains 0 U/ml, standard 2 contains 25 U/ml and so on with standard 6...

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Thank you for the CA19.9 ELISA kits (cat: ab108642) we have now safely received them. I just had one question, on these kits it doesn’t specify the expiration date of the kits or the reagents. Could you please send me the expiration date of these kits ...

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Thank you for your inquiry. This kit has a shelf life of 1 year.  For the lots that you ordered, we would expect them to be good until October 2012 when stored properly. I hope this information helps.  Please contact us with any other questions.

Dear technical team,   Our customer would like to purchase these kits and wants to know the meaning of "unit"  in Sensitivity : 5U/ml.   1. unit definition   2. is it possible conversion of unit  to  ng?   Check this please.     Best regards,

Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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