Overview

  • Product name

    Human Carbonic Anhydrase 2 ELISA Kit
    See all Carbonic anhydrase 2/CA2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 9.3%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.05 ng/ml
  • Range

    0.063 ng/ml - 4 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rabbit, Cow
  • Product overview

    The Human CA2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222881) is designed for detection of human CA2 in plasma, serum, and cell culture samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CA2 in approximately 4 hours. A polyclonal antibody specific for human CA2 has been pre-coated onto a 96-well microplate with removable strips. CA2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CA2, which is recognized by a Streptavidin-Peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Carbonic anhydrase 2 (CA2, CAC), also known as carbonate dehydratase II and carbonic anhydrase II, belongs to the alpha-carbonic anhydrase family. It contains 259 amino acid residues and has a molecular mass of 29 kDa. CA2 catalyzes reversible hydration of carbon dioxide and is expressed at high levels in osteoclasts during bone resorption. CA2 supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    40X Biotinylated Human CA2 1 x 150µl
    Anti- Human CA2 coated Microplate (12 x 8 wells) 1 unit
    Chromogen Substrate 1 x 8ml
    Human CA2 Standard 1 vial
    Sealing Tapes 1 x 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
  • Involvement in disease

    Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
  • Sequence similarities

    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • CA 2
    • CA II
    • CA-II
    • Ca2
    • CAC
    • CAH2_HUMAN
    • CAII
    • Car 2
    • Car2
    • Carbonate dehydratase II
    • Carbonic anhydrase 2
    • Carbonic anhydrase B
    • Carbonic anhydrase C
    • Carbonic anhydrase C, formerly
    • Carbonic anhydrase II
    • Carbonic dehydratase
    • epididymis luminal protein 76
    • Epididymis secretory protein Li 282
    • HEL-76
    • HEL-S-282
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human CA2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab222881 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222881.
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