• Product name

    Human Carcino Embryonic Antigen ELISA Kit (CEA)
    See all Carcino Embryonic Antigen CEA kits
  • Detection method

  • Sample type

  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1 ng/ml
  • Range

    1 ng/ml - 120 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Carcino Embryonic Antigen in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Carcino Embryonic Antigen in Human serum.

    A 96-well plate has been precoated with anti-Carcino Embryonic Antigen antibodies. Samples and standards and are added to the wells, where Carcino Embryonic Antigen in the sample and standards binds to the precoated antibody. Added Anti-Carcino Embryonic Antigen-HRP conjugate binds to the antibody-Carcino Embryonic Antigen complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Carcino Embryonic Antigen in the sample and the intensity is measured at 450 nm.

    Get higher sensitivity in only 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.


  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Carcino Embryonic Antigen Coated Microplate (12 x 8 wells) 1 unit
    Anti-Carcino Embryonic Antigen HRP Conjugate 1 x 13ml
    CEA Standard 0 - 0 ng/mL 1 x 1ml
    CEA Standard 1 - 3 ng/mL 1 x 1ml
    CEA Standard 2 - 12 ng/mL 1 x 1ml
    CEA Standard 3 - 30 ng/mL 1 x 1ml
    CEA Standard 4 - 60 ng/mL 1 x 1ml
    CEA Standard 5 - 120 ng/mL 1 x 1ml
    Stop Solution 1 x 11ml
    TMB Reagent 1 x 11ml
  • Research areas

  • Relevance

    Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
  • Cellular localization

    Cell Membrane
  • Alternative names

    • Carcinoembryonic antigen
    • Carcinoembryonic antigen related cell adhesion molecule 5
    • CD66e
    • CD66e antigen
    • CEA
    • CEACAM 5
    • CEACAM5
    • DKFZp781M2392
    • Meconium antigen 100
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab108635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.


  • Representative standard curve using ab108635.s



ab108635 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your new enquiry and continuous interest in our products.

I have conducted a search for you and we have FITC and PE-conjugated antibodies but not HRP-linked primary antibody against CEA.

However, we have two CEA ELISA kits in our catalogue (ab99992, ab108635) which you may be interested. Please take a look at the on-line datasheet for further information and le me know if you have any further questions.

Have a nice day!

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Thank you for your e-mail. We do not have experience with the determination of CEA in mouse with our CEA ELISA, ab108635. It could work but I cannot give you a guarantee.

In this case I would be happy to offer the same testing discount promotion as discussed re: ab99992.

Please let me know if you are interested.

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Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your inquiry. Average Recovery (from 6 experiments) = 95.9% The minimum detectable sensitivity of ab108635 as measured by 2 SD from the mean of a 0.5, 0.75, 1, and 3 ng/mL calibrator is estimated to be 1.0 ng/mL. I hope this information is helpful. Please let me know if I can be of any further assistance.

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