Overview

  • Product name

    Human Carcino Embryonic Antigen ELISA Kit (CEA)
    See all Carcino Embryonic Antigen CEA kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.2 ng/ml
  • Range

    0.343 ng/ml - 250 ng/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116.7 93% - 136%
    Serum 145.6 135% - 156%
    Plasma 103.8 82% - 124%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    1,000X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CEA (lyophilized) 2 vials
    CEA Microplate (12 x 8 well strips) 1 unit
    Recombinant Human CEA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
  • Cellular localization

    Cell Membrane
  • Alternative names

    • Carcinoembryonic antigen
    • Carcinoembryonic antigen related cell adhesion molecule 5
    • CD66e
    • CD66e antigen
    • CEA
    • CEACAM 5
    • CEACAM5
    • DKFZp781M2392
    • Meconium antigen 100
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab99992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • CEA measured in biological fluids showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab99992.

  • Representative Standard Curve using ab99992.

Protocols

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that the kit should contain 8 ul of HRP-Streptavidin Concentrate (Item G). Please tell your customer to spin down the vial. The kit was ordered in November 2011 and we did change the amount of HRP-Streptavidin Concentrate (Item G) to 200ul in March 2012.

The protocol booklet that your customer received with this kit will detail that HRP-Streptavidin Concentrate (Item G) is 8ul.

I would also like to mention that the Abpromise guarantee period for our products is 6 month and therefore we suggest to check the products shortly after they delivered to avoid disappointment.

I attached the correct protocol for the kit your customer received to this email for your information.

I hope this will be helpful to your customer and wish you both a

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CEA concentration of normal or lung cancer sera

Average Excellent 5/5 (Ease of Use)
Abreviews
Difference between normal group and lung adenocarcinoma (ADC) group was very clear.

But the absolute values measured by ab99992 were remarkably higher than clinical tests in hospital or CRO companies.

To make matters worse, when we increased the dilution factor (x2, x5, or x10 dilution), the absolute values of CEA also gradually increased.

Hence this kit can be used for only "relative quantification" studies.

Abcam user community

Verified customer

Submitted Nov 05 2013

Answer

Thank you for your inquiry.

The capture antibody in this kit is supplied by an outside lab and unfortunately they were not willing to provide me with any other details about it. They did want to know if you were interested in it to follow up on some data from the ELISA assay? Perhaps they would be willing to offer it for sale, but I'm not sure if that's what you wanted.

I hope this information helps. Please contact us with any other question.

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Answer

Thank you for your new enquiry and continuous interest in our products.

I have conducted a search for you and we have FITC and PE-conjugated antibodies but not HRP-linked primary antibody against CEA.

However, we have two CEA ELISA kits in our catalogue (ab99992, ab108635) which you may be interested. Please take a look at the on-line datasheet for further information and le me know if you have any further questions.

Have a nice day!

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Question
Answer

Unfortunately there is no cross reactivity data available testing the Human CEA ELISA Kit ab99992 with mouse. If it helps though, the capture antibody is a mouse monoclonal and reacts with the protein determinant belonging to the “gold” epitope group 4. The detection antibody is a mouse monoclonal antibody reacting to the “gold” epitope group 5.

We do have a testing discount promotion which you may be interested in as you will be testing mouse with this ELISA kit. Please see below for a description:

===================

To our knowledge, ab99992 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab99992 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free ELISA kit or 20% off a primary antibody or protein product.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab99992 in mouse. I will then send a discount code. This code must be issued before purchasing ab99992 so please wait for my reply before ordering.

2. Purchase ab99992 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any ELISA kit or 20% off a primary antibody or protein product and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab99992 turns out to be unsuitable for 1ELISA kit or 20% off a primary antibody or protein product, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

At the moment we do not sell kit components separately, this is because that we only produce enough reagents for use with the kit and do not have any spare to sell separately. We may in future add the antibody in its own right but for now it can only be purchased as part of a kit.

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Answer

Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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