Overview

  • Product name

    Human Cardiac Troponin I ELISA Kit
    See all Cardiac Troponin I kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human heart 8 3%
    Inter-assay
    Sample n Mean SD CV%
    Human heart 3 7.1%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.4 pg/ml
  • Range

    31.3 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97 93% - 101%
    Tissue Extracts 112 104% - 118%
    Cell culture media 102 97% - 108%
    Heparin Plasma 107 99% - 117%
    EDTA Plasma 93 90% - 97%
    Citrate Plasma 93 87% - 100%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    As of November 7, 2018, Human Cardiac Troponin 1 SimpleStep ELISA® kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency when quantifying Cardiac Troponin 1 protein in human serum, plasma, cell culture supernatant, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Sample Diluent NS: 4.4 pg/mL
    Sample Diluent 50BP: 8.8 pg/mL
    1X Cell Extraction Buffer PTR: 10.7 pg/mL

  • Notes

    The regulatory troponin complex regulates skeletal and cardiac muscle contraction. This complex, together with tropomyosin, is located on the actin filament and it is composed of three protein subunits: troponin T (the tropomyosin-binding subunit), troponin I (the inhibitory subunit, which inhibits the ATPase activity of acto-myosin), and troponin C (the Ca2+-binding subunit). Troponins T and I have unique cardiac isoforms, whereas cardiac and skeletal muscle share troponin C. Specifically, three human troponin I isoforms have been described: one is expressed in cardiac muscle (Cardiac Troponin I) and the other two are found in slow-twitch and fast-twitch skeletal muscle fibers (slow sTnI and fast sTnI, respectively). The sequence homology between Cardiac Troponin I and slow sTnI is approximately 40% and somewhat less for fast sTnI. Cardiac Troponin I is 209 amino acid long with a molecular weight of approximately 24 kDa. Mouse and rat Cardiac Troponin I proteins both show 93% amino acid identity to human Cardiac Troponin I. The presence of human Cardiac Troponin I in serum (together with chest pain and electrocardiographic changes) is now considered as one highly specific biochemical marker of myocardial injury, risk stratification of acute coronary syndrome and myocardial infarction. Mutations of Cardiac Troponin I are associated with hereditary cardiomyopathy. Specifically, defects in Cardiac Troponin I are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intra-familial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in Cardiac Troponin I also cause cardiomyopathy familial restrictive type 1 (RCM1). RCM1 is a heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Furthermore, cardiomyopathy dilated type 2A (CMD2A) and cardiomyopathy dilated type 1FF (CMD1FF), disorders characterized by ventricular dilation and impaired systolic function resulting in congestive heart failure and arrhythmia, are caused by defects in Cardiac Troponin I.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Cardiac Troponin I Capture Antibody 1 x 600µl
    10X Human Cardiac Troponin I Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human Cardiac Troponin I Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Cellular localization

    Cytosol; Sarcomere.
  • Alternative names

    • Cardiomyopathy dilated 2A (autosomal recessive)
    • CMD1FF
    • CMD2A
    • CMH7
    • cTnI
    • RCM1
    • TNNC1
    • TNNI3
    • Troponin I cardiac muscle
    • Troponin I type 3 (cardiac)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab200016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between current human Cardiac Troponin 1 SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

  • The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The Cardiac Troponin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Cardiac Troponin 1 were measured in duplicate and interpolated from the Cardiac Troponin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin 1 concentration was determined to be 1,793 pg/mL in human heart tissue extract.

  • Human heart was analyzed based on a 10 µg/mL extract load while human skeletal muscle and human brain extracts were analyzed based on a 200 µg/mL extract load. The concentrations of Cardiac Troponin 1 were measured in duplicate and interpolated from the Cardiac Troponin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin 1 concentration was determined to be 182.4 ng/mg in human heart tissue extract and undetectable in human skeletal muscle and brain tissue extracts.

Protocols

References

This product has been referenced in:

  • Zhang Z  et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). Read more (PubMed: 29977885) »
  • Yu J  et al. Abnormal expression of miR-133a in patients with acute myocardial infarction following radical surgery for gastric cancer and the underlying mechanism. Mol Med Rep 18:5023-5029 (2018). Read more (PubMed: 30320356) »
See all 2 Publications for this product

Customer reviews and Q&As

Human Urine

Excellent Excellent 5/5 (Ease of Use)
Abreviews
ab200016 - Human Cardiac Troponin I ELISA Kit works with the sample type human urine.

Abcam user community

Verified customer

Submitted Feb 14 2018

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