Human Caspase-1 Matched Antibody Pair Kit (ab222264)
- Datasheet
- References
- Protocols
Overview
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Product nameHuman Caspase-1 Matched Antibody Pair Kit
See all Caspase-1 kits -
Detection methodColorimetric
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Assay typeELISA set
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Range15.6 pg/ml - 1000 pg/ml
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Species reactivityReacts with: Human
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Product overview
Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Caspase-1.
Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.
- Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.
- Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.
- Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.
Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab219633) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
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Tested applicationsSuitable for: ELISAmore details
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PlatformReagents
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 10 x 96 tests 2 x 96 tests Human Caspase-1 Capture Antibody 1 x 100µg 1 x 20µg Human Caspase-1 Detector Antibody 1 x 25µg 1 x 5µg Human Caspase-1 Lyophilized Protein 1 vial 1 vial -
Research areas
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FunctionThiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
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Tissue specificityExpressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
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Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism. -
Cellular localizationCytoplasm.
- Information by UniProt
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Alternative names
- CASP-1
- CASP1
- CASP1_HUMAN
see all -
Database links
- Entrez Gene: 834 Human
- Omim: 147678 Human
- SwissProt: P29466 Human
- Unigene: 2490 Human
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab222264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Human Caspase-1 standard curveStandard calibration curve. Background subtracted values are graphed.
Protocols
References
ab222264 has not yet been referenced specifically in any publications.
