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    human-caspase-3-elisa-kit-ab285337.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Human Caspase-3 ELISA Kit (ab285337)

  • Datasheet
  • Protocol Booklet
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Caspase-3 (Human) ELISA Kit Standard Curve

    Key features and details

    • Sensitivity: 0.188 ng/ml
    • Range: 0.313 ng/ml - 20 ng/ml
    • Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Caspase-3 ELISA Kit
    • Detection method

      Colorimetric
    • Sample type

      Serum, Plasma, Other biological fluids, Tissue Homogenate
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 0.188 ng/ml
    • Range

      0.313 ng/ml - 20 ng/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Human Caspase-3 ELISA Kit (ab285337, K4221) is a sandwich ELISA assay for the quantitative measurement of human Caspase-3 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Caspase-3 captured from the samples.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K4221 Caspase-3 (Human) ELISA Kit. K4221-100 is the same size as the 96 test size of ab285337.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 96 tests
      25X Wash buffer 1 x 30ml
      Antibody dilution buffer 1 x 10ml
      Biotin-Detection antibody (Concentrated) 1 x 120µl
      HRP-Streptavidin Conjugate (SABC) 1 x 120µl
      Lyophilized Standard 2 vials
      Micro ELISA Plate 1 unit
      Plate Sealer 5 units
      SABC dilution buffer 1 x 10ml
      Sample and Standard Dilution Buffer 1 x 20ml
      Stop Solution 1 x 10ml
      TMB Substrate 1 x 10ml
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Caspases etc
      • Caspases
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Caspases
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Other proteases
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Caspases
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism
    • Database links

      • Entrez Gene: 836 Human

      Images

      • Caspase-3 (Human) ELISA Kit Standard Curve
        Caspase-3 (Human) ELISA Kit Standard Curve

        Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab285337? Please let us know so that we can cite the reference in this datasheet.

      ab285337 has not yet been referenced specifically in any publications.

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