Overview

  • Product name

    Human Caspase-9 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 6.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 9%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.4 ng/ml
  • Range

    1.6 ng/ml - 100 ng/ml
  • Recovery

    103 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Caspase-9 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Caspase-9 concentrations in cell lysate, cell culture supernatant and serum.

    Caspase-9 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Caspase-9 detection antibody and the microplate is then incubated at room temperature. Following washing a HRP conjugated anti-rabbit-IgG antibody is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Caspase-9 amount of sample captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Anti-rabbit-IgG HRP conjugated antibody 1 x 10µl
    Human Caspase-9 Standard 2 vials
    10X Lysis buffer 1 x 15ml
    Microplate coated with monoclonal antibody to Human Caspase-9 (12 x 8 wells) 1 unit
    Polyclonal anti-Human Caspase-9 Detection Antibody (rabbit) 1 x 70µl
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • Research areas

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Alternative names

    • APAF-3
    • APAF3
    • Apoptotic protease Mch-6
    • Apoptotic protease-activating factor 3
    • CASP-9
    • CASP9
    • CASP9_HUMAN
    • Caspase 9 apoptosis related cysteine peptidase
    • Caspase-9 subunit p10
    • ICE-LAP6
    • ICE-like apoptotic protease 6
    • MCH6
    • PPP1R56
    • Protein phosphatase 1 regulatory subunit 56
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119508.

Protocols

References

This product has been referenced in:

  • Chen H  et al. Poly (I:C) transfection induces mitochondrial-mediated apoptosis in cervical cancer. Mol Med Rep 13:2689-95 (2016). Read more (PubMed: 26848042) »
See 1 Publication for this product

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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