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|Sample type||Average %||Range|
|Cell culture media||104||96% - 116%|
|Goat Serum||113||105% - 126%|
Abcam’s Catalase in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Catalase protein in Catalase in human cell and tissue lysates.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Quantify as low as 43 pg/ml of Human Catalase, in just 90 minutes.
This kit is knockout validated for specifically detecting catalase in human HAP1 cells.
Catalase is the classical marker for peroxisomes. Catalase occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Catalase promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Catalase is encoded by the CAT gene, defects in CAT are the cause of acatalasia (ACATLAS), also known as acatalasemia. This disease is characterized by absence of Catalase activity in red cells and is often associated with ulcerating oral lesions.
|Components||1 x 96 tests|
|10X Catalase Capture Antibody||1 x 600µl|
|10X Catalase Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR (ab193970)||1 x 10ml|
|Antibody Diluent 5B||1 x 6ml|
|Catalase Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS||1 x 12ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab171572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background subtracted data from duplicate measurements are plotted.
Background subtracted data from duplicate measurements are plotted. Due to relative high expression levels of catalase in the cell line HEK 293, signal saturates when lysate is loaded at > 16 µg/mL.
Interpolated values of catalase are plotted for the indicated cell lines based on an extract load of 16 µg/mL.
Raw data is plotted for wild-type and catalase-knockout HAP1 cell extracts tested at 50 μg/mL.
Immunocytochemical staining of Human HeLa cells using the catalase detector antibody in this kit (ab110292). The target protein locates mainly in peroxisomes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"