Overview

  • Product name

    Human Catalase ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa lysates 5 3%
    Inter-assay
    Sample n Mean SD CV%
    HeLa lysates 3 3.1%
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    43 pg/ml
  • Range

    0.31 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 104 96% - 116%
    Goat Serum 113 105% - 126%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Catalase in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Catalase protein in Catalase in human cell and tissue lysates.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Quantify as low as 43 pg/ml of Human Catalase, in just 90 minutes.


    This kit is knockout validated for specifically detecting catalase in human HAP1 cells.

  • Notes

    Catalase is the classical marker for peroxisomes. Catalase occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Catalase promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Catalase is encoded by the CAT gene, defects in CAT are the cause of acatalasia (ACATLAS), also known as acatalasemia. This disease is characterized by absence of Catalase activity in red cells and is often associated with ulcerating oral lesions.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab171572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background subtracted data from duplicate measurements are plotted.

  • Background subtracted data from duplicate measurements are plotted. Due to relative high expression levels of catalase in the cell line HEK 293, signal saturates when lysate is loaded at > 16 µg/mL.

  • Interpolated values of catalase are plotted for the indicated cell lines based on an extract load of 16 µg/mL.

  • Raw data is plotted for wild-type and catalase-knockout HAP1 cell extracts tested at 50 μg/mL.

  • Immunocytochemical staining of Human HeLa cells using the catalase detector antibody in this kit (ab110292). The target protein locates mainly in peroxisomes.

Protocols

References

This product has been referenced in:

  • Liu CW  et al. Temporal expression profiling of plasma proteins reveals oxidative stress in early stages of Type 1 Diabetes progression. J Proteomics 172:100-110 (2018). Read more (PubMed: 28993202) »
  • Wang G  et al. Significance of Lipid-Derived Reactive Aldehyde-Specific Immune Complexes in Systemic Lupus Erythematosus. PLoS One 11:e0164739 (2016). Read more (PubMed: 27749917) »
See all 2 Publications for this product

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