Key features and details
- Sensitivity: 5 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Cathepsin B ELISA Kit
See all Cathepsin B kits
Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range156 pg/ml - 10000 pg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Human Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).
A Cathepsin B specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin B amount of sample captured in plate.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Cathepsin B antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Cathepsin B antibody 1 x 100µl Lyophilized recombinant Human Cathepsin B standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence similaritiesBelongs to the peptidase C1 family.
Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Amyloid precursor protein secretase
- APP secretase
Cathepsin B measured in culture supernatants from control (-) or stimulated cells cells (P/L: 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours; PHA: 48 hours with 2% PHA-M; LifeTechnologies). Data shown represents duplicates (+/- SD), samples tested in the dilution range of 1/1-1/20.
Cathepsin B measured in biological fluids (tested in the range of 1/10-1/1000; duplicates +/- SD).
Representative Standard Curve Using ab119584.
ab119584 has been referenced in 6 publications.
- Thangavelu B & Boutté AM Single Molecule Assay for Ultrasensitive Detection of Cathepsin B in Human Blood. ACS Omega 6:9609-9616 (2021). PubMed: 33869941
- Fujimoto T et al. Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes. Oncol Lett 21:30 (2021). PubMed: 33240436
- Dayde D et al. Identification of Blood-Based Biomarkers for the Prediction of the Response to Neoadjuvant Chemoradiation in Rectal Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34298853
- Rong X et al. Chronic Periodontitis and Alzheimer Disease: A Putative Link of Serum Proteins Identification by 2D-DIGE Proteomics. Front Aging Neurosci 12:248 (2020). PubMed: 32973486
- Sharma S et al. Evaluation of Quantitative Relationship Between Target Expression and Antibody-Drug Conjugate Exposure Inside Cancer Cells. Drug Metab Dispos 48:368-377 (2020). PubMed: 32086295
- Zhu S et al. Inhibiting autophagy potentiates the anticancer activity of IFN1@/IFNa in chronic myeloid leukemia cells. Autophagy 9:317-27 (2013). PubMed: 23242206