Overview

  • Product name

    Human Cathepsin D ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human Cathepsin D in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin D in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin D specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin D is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin D amount of sample captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Cathepsin D antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Cathepsin D antibody 1 x 130µl
    Lyophilized recombinant Human Cathepsin D standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Amber Bottle 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Involvement in disease

    Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Cellular localization

    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Alternative names

    • CATD_HUMAN
    • Cathepsin D heavy chain
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Lysosomal aspartyl protease
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Cathepsin D measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD). We recommend to test the human plasma, serum and urine samples in the range of 1:20-1:500, the saliva in the range of 1:100-1:1000 and the cell culture supernatants 1:1-1:1000.

  • Representative Standard Curve using ab119586.

Protocols

References

This product has been referenced in:

  • Braconi D  et al. Inflammatory and oxidative stress biomarkers in alkaptonuria: data from the DevelopAKUre project. Osteoarthritis Cartilage N/A:N/A (2018). ELISA . Read more (PubMed: 29852277) »
  • Vital P  et al. The senescence-associated secretory phenotype promotes benign prostatic hyperplasia. Am J Pathol 184:721-31 (2014). Read more (PubMed: 24434012) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your call today and for letting us know about the trouble with this kit.

Please keep me updated about the results with the modifications that we discussed, and let me know if there is anything else that we can do for you.



Thank you again, and have a nice day.

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Answer

Thank you for your patience.
I received the following reply:
Either washing or blotting is effective. In this assay, blotting works the best and it is validated. Please follow the protocol.
I hope this information is helpful to you, and I wish you good luck.
Please do not hesitate to contact us if you need any more advice or information.
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