Overview

  • Product name

    Human Cathepsin S ELISA Kit (CTSS)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 4 pg/ml
  • Range

    4 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 126.3 106% - 138%
    Plasma = 127.8 115% - 140%
    Cell culture media = 128.5 104% - 139%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Cathepsin S (CTSS) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Cathepsin S in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Cathepsin S (CTSS) coated on a 96-well plate. Standards and samples are pipetted into the wells and Cathepsin S present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cathepsin S antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cathepsin S bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155427 -  Assay Diluent A

  • Representative standard curve using ab155427 - Assay Diluent B

Protocols

References

ab155427 has not yet been referenced specifically in any publications.

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