Human CBL knockout HEK-293T cell pellet (ab278825)

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Western blot - Human CBL knockout HEK293T cell pellet (ab278825)
  • Sanger Sequencing - Human CBL knockout HEK293T cell pellet (ab278825)

Overview

  • Product name

    Human CBL knockout HEK-293T cell pellet
    See all CBL kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon9.

  • Passage number

    <20

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

  • Pathway

    Protein modification; protein ubiquitination.

  • Involvement in disease

    Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.

  • Sequence similarities

    Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.

  • Domain

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • 4732447J05Rik
    • C CBL
    • Cas Br M (murine) ecotropic retroviral transforming sequence
    • Casitas B lineage lymphoma proto oncogene
    • Casitas B-lineage lymphoma proto-oncogene
    • cbl
    • CBL 2
    • CBL_HUMAN
    • CBL2
    • E3 ubiquitin protein ligase CBL
    • E3 ubiquitin-protein ligase CBL
    • Oncogene CBL2
    • Proto oncogene c CBL
    • Proto-oncogene c-CBL
    • RGD1561386
    • RING finger protein 55
    • RNF55
    • Signal transduction protein CBL
    see all

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab278825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.

Images

  • Western blot - Human CBL knockout HEK293T cell pellet (ab278825)
    Western blot - Human CBL knockout HEK293T cell pellet (ab278825)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:CBL knockout HEK293T cell lysate (20 ug)
    Lane 3:THP-1 cell lysate (20 ug)
    Lane 4:Raji cell lysate (20 ug)

    ab32027 was shown to specifically react with CBL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267245 (knockout cell lysate ab257200) was used. Wild-type and CBL knockout samples were subjected to SDS-PAGE. ab32027 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human CBL knockout HEK293T cell pellet (ab278825)
    Sanger Sequencing - Human CBL knockout HEK293T cell pellet (ab278825)
    Homozygous: 10 bp deletion in exon9

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab278825? Please let us know so that we can cite the reference in this datasheet.

ab278825 has not yet been referenced specifically in any publications.

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