Overview

  • Product name

    Human CCL18 Matched Antibody Pair Kit
    See all CCL18 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    3.1 pg/ml - 200 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human CCL18
    .


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab211649) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human CCL18 Capture Antibody 1 x 100µg 1 x 20µg
    Human CCL18 Detector Antibody 1 x 25µg 1 x 5µg
    Human CCL18 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
  • Tissue specificity

    Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Alternative macrophage activation associated CC chemokine 1
    • Alternative macrophage activation-associated CC chemokine 1
    • AMAC-1
    • AMAC1
    • CC chemokine PARC
    • CCL18
    • CCL18(4-69)
    • CCL18_HUMAN
    • CKb7
    • DC-CK1
    • DCCK1
    • Dendritic cell chemokine 1
    • Macrophage inflammatory protein 4
    • MIP-4
    • MIP4
    • PARC
    • Pulmonary and activation regulated chemokine
    • Pulmonary and activation-regulated chemokine
    • SCYA18
    • Small inducible cytokine A18
    • Small-inducible cytokine A18
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab219527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab219527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219527.
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