Overview

  • Product name

    Human CCL23 ELISA Kit
    See all CCL23 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 8.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.5%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.54 pg/ml
  • Range

    7.03 pg/ml - 450 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91 89% - 92%
    Serum 106 98% - 117%
    Other biological fluids 93 88% - 96%
    Heparin Plasma 98 88% - 106%
    EDTA Plasma 101 98% - 106%
    Citrate Plasma 105 93% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    CCL23 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CCL23 protein in human serum, plasma, cell culture supernatant, and synovial fluid. 


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    CCL23 (Macrophage inflammatory protein 3) is a chemokine involved in the recruitment of monocytes, neutrophils and resting T cells. CCL23 functions by inhibition myeloid progenitor cell proliferation. CCL23 is found at high levels in the synovial fluid of patients with rheumatoid arthritis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CCL23 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human CCL23 Capture Antibody (Lyophilized) 1 vial
    Human CCL23 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
  • Tissue specificity

    High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C C motif chemokine ligand 23
    • C-C motif chemokine 23
    • C6 beta chemokine
    • CCL 23
    • CCL23
    • CCL23(30-99)
    • CCL23_HUMAN
    • Chemokine (C C motif) ligand 23
    • Chemokine CC motif ligand 23
    • ck beta 8
    • CK-beta-8
    • CKB 8
    • CKB-8
    • Ckb-8-1
    • CKB8
    • Hmrp-2a
    • Macrophage inflammatory protein 3
    • MIP 3
    • MIP-3
    • MIP3
    • MPIF 1
    • MPIF-1
    • MPIF1
    • Myeloid progenitor inhibitory factor 1
    • OTTHUMP00000163948
    • SCYA 23
    • SCYA23
    • Small inducible cytokine A 23
    • Small inducible cytokine subfamily A (Cys-Cys), member 23
    • Small inducible cytokine subfamily A, member 23
    • Small-inducible cytokine A23
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab216169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 698 pg/mL in serum, 621 pg/mL in plasma (citrate), 689 pg/mL in plasma (heparin) and 592 pg/mL in plasma (EDTA).

  • The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: synovial fluid 25% and THP-1 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 182 pg/mL in synovial fluid and 130 pg/mL in THP-1 supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 797 pg/mL with a range of 229 – 1,754 pg/mL.

Protocols

References

ab216169 has not yet been referenced specifically in any publications.

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