Overview

  • Product name

    Human CCL27 ELISA Kit (CTACK)
    See all CCL27 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 8.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 7.5%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    9 pg/ml
  • Range

    94 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 113 104% - 129%
    Cell culture media 89 86% - 94%
    Hep Plasma 85 73% - 98%
    EDTA Plasma 89 86% - 94%
    Cit plasma 113 109% - 118%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Goat, Cow, Pig
  • Product overview

    Abcam’s CCL27 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CCL27 protein in Human serum, plasma and cell culture supernatants.

     The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity: Samples diluted in Sample Dilunet NS - 9 pg/mL

                        Samples diluted in Sample Diluent 25BS - 19 pg/mL

  • Notes

    CC chemokine ligand 27 (CCL27) is a member of a superfamily of small secreted proteins that attract circulating lymphocytes to an area of injury/infection by binding with G protein-coupled receptors expressed on the migrating target cells. CCL27 is expressed in the skin and selectively attracts CLA+ memory T-cells into the skin microenvironment. CCL27 expression can be up-regulated by the proinflammatory cytokines TNF-α and IL-1β increasing migration of memory T-cells to the skin during inflammation. In normal skin suprabasal keratinocytes express minute amounts of CCL27; where as in lesions of human Atopic Dermatitis, Contact Dermatitis, and psoriasis, the keratinocytes demonstrate stronger expression of CCL27 which is thought to attract pro-inflammatory CLA+ memory T-cells to the lesions contributing to the inflammatory process and perpetuates the disease.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CCL27 Capture Antibody 1 x 600µl
    10X Human CCL27 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human CCL27 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
  • Tissue specificity

    Testis, thymus, placenta, ovary and skin.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ALP
    • C-C motif chemokine 27
    • CC chemokine ILC
    • Ccl27
    • CCL27_HUMAN
    • CTACK
    • CTAK
    • Cutaneous T cell attracting chemokine
    • Cutaneous T-cell-attracting chemokine
    • ESkine
    • IL 11 R alpha locus chemokine
    • IL 11 Ralpha locus chemokine
    • IL-11 R-alpha-locus chemokine
    • ILC
    • Ligand 27
    • PESKY
    • Scya 27
    • SCYA27
    • Skinkine
    • Small inducible cytokine A27
    • Small inducible cytokine subfamily A (Cys-Cys), member 27
    • Small-inducible cytokine A27
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab200013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Background subtracted data from duplicate measurements are plotted. Serum samples from a total of 10 individual Normal serum donors and 3 donors diagnosed with Atopic Dermatitis (AD) were assayed. Complete medical histories for all donors is not known.

Protocols

References

ab200013 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200013.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up