Product nameHuman CCL28 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 25 pg/ml
Range27.4 pg/ml - 20000 pg/ml
> 65 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 67.7 59% - 81% Serum 70.6 60% - 98% Plasma 68.6 59% - 82%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CCL28 (lyophilized) 2 vials CCL28 Microplate (12 strips x 8 wells) 1 x 200µl Recombinant Human CCL28 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceThis protein belongs to the subfamily of small cytokine CC proteins. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. This cytokine displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils and binds to chemokine receptors CCR3 and CCR10. It induces calcium mobilization in a dose-dependent manner. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs.
- CCK 1
- CCL 28
- SwissProt: Q9NRJ3 Human
Our Abpromise guarantee covers the use of ab99988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Huang G et al. Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells. Sci Rep 6:27152 (2016). Read more (PubMed: 27250766) »
- Yoon JH et al. Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood. Transfusion 55:1008-12 (2015). Read more (PubMed: 25412854) »