Overview

  • Product name

    Human CCL28 Matched Antibody Pair Kit
    See all CCL28/MEC kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    15.62 pg/ml - 1000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant Human CCL28.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab218263) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human CCL28 Capture Antibody 1 x 100µg 1 x 20µg
    Human CCL28 Detector Antibody 1 x 25µg 1 x 5µg
    Human CCL28 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
  • Tissue specificity

    Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C C motif chemokine ligand 28
    • C-C motif chemokine 28
    • CC chemokine CCL28
    • CCK 1
    • CCK1
    • CCK1 protein
    • CCL 28
    • CCL28
    • CCL28_HUMAN
    • Chemokine (C-C motif) ligand 28
    • chemokine (C-C motif) ligand 28 splice variant chi
    • MEC
    • Mucosae associated epithelial chemokine
    • Mucosae-associated epithelial chemokine
    • Protein CCK1
    • SCYA28
    • Small inducible cytokine A28 [Precursor]
    • small inducible cytokine subfamily A (Cys-Cys), member 28
    • Small-inducible cytokine A28
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab220141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab220141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab220141.
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