Overview

  • Product name

    Human CD105 ELISA Kit
    See all CD105 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 3 1.8%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 5 3%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11.1 pg/ml
  • Range

    78.125 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 84% - 120%
    Cell culture extracts 103 99% - 110%
    Tissue Extracts 106 96% - 119%
    Cell culture media 107 95% - 116%
    Heparin Plasma 88 71% - 113%
    EDTA Plasma 100 84% - 119%
    Citrate Plasma 80 74% - 84%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Cow
  • Product overview

    CD105 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD105 protein in human serum, plasma, cell culture supernatant, and cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in 1X Cell Extraction Buffer PTR + Enhancer - 11.1 pg/mL


    Samples in Sample Diluent NS + Enhancer - 17.5 pg/mL

  • Notes

    CD105 is a major glycoprotein of vascular endothelium. CD105 is involved in the regulation of angiogenesis. It may play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 acts as TGF-beta co-receptor and is involved in the TGF-beta/BMP signaling cascade. It is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and it modulates TGF-beta1 signaling through SMAD3. CD105 is a homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Circulating soluble form of CD105 is elevated in patients with preeclampsia, atherosclerosis and certain cancers including breast, colorectal, and myeloid malignancies.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab217773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human CD105 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD105 were measured in duplicates, interpolated from the CD105 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.225%, plasma (citrate) 2.225%, plasma (heparin) 2.225% and plasma (EDTA) 2.225%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 94,646 pg/mL in neat serum, 71,971 pg/mL in neat plasma (citrate), 75,408 pg/mL in neat plasma (heparin), and 76,842 pg/mL in neat plasma (EDTA).

  • Interpolated concentrations of native CD105 in HUVEC cell extract based on a 40 µg/mL extract load, human placenta tissue extract based on a 90 µg/mL extract load, and human heart tissue extract based on a 60 µg/mL extract load. The concentrations of CD105 were measured in duplicate and interpolated from the CD105 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 2548 pg/mL in HUVEC cell extract, 542.9 pg/mL in placenta tissue extract, and 528.1 pg/mL in heart tissue extract.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 61,333 pg/mL with a range of 30,103 – 91,256 pg/mL.

  • Serum from 10 healthy (not pregnant) female individuals, 5 healthy females in the third trimester of pregnancy (pooled), and 1 individual female in the third trimester showing signs of mild pre-eclampsia were tested for levels of CD105. The concentrations of CD105 were measured from serum in samples in 3 different dilutions in duplicates and interpolated from the CD105 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean CD105 concentration was determined to be 61,333 pg/mL in healthy female donor serum, 30,8945 pg/mL in healthy pregnant donor serum, and 703,119 pg/mL in pre-eclampsia patient serum.

Protocols

References

ab217773 has not yet been referenced specifically in any publications.

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