Overview

  • Product name

    Human CD105 ELISA Kit (Endoglin)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    12.35 pg/ml - 3000 pg/ml
  • Recovery

    > 96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.92 87% - 110%
    Serum 120.1 111% - 130%
    Plasma 116.7 94% - 132%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CD105 (Endoglin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD105 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD105 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD105 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD105 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD105 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100507.

Protocols

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry.

ab100507 CD105 human ELISA kit has been validated with plasma and is compatible with EDTA, heparin, and citrate anti-coagulants unless otherwise stated in the Introduction section of the manual. Any one from the above can be used with this ELISA kit.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your message and I'm sorry to hear you have been having some difficulties.

Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of samples you are using, and I can find out some recommended dilutions for you for these kits.

Thank you for your time. i look forward to hearing from you.

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