Overview

  • Product name

    Human CD127 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 2.8ng/ml 0.168 6%
    2 16 8ng/ml 0.36 4.5%
    3 16 14.3ng/ml 0.815 5.7%
    Inter-assay
    Sample n Mean SD CV%
    1 24 3.4ng/ml 0.245 7.2%
    2 24 9.1ng/ml 0.51 5.6%
    3 24 16.2ng/ml 1.07 6.6%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    312 pg/ml - 20000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human CD127 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213799) is designed for the quantitative detection of Human CD127 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD127 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD127 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD127 amount of sample captured in plate.

  • Notes

    CD127 (also known as interleukin-7 receptor or IL-7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukin 7 (IL-7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL-2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukin 2, 4, 7, 9, and 15. CD127 has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D) J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, CD127 antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human CD127 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human CD127 antibody 1 x 130µl
    Lyophilized recombinant Human CD127 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
  • Involvement in disease

    Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications

    N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • CD 127
    • CD127
    • CD127 antigen
    • CDw127
    • IL 7R
    • IL 7R alpha
    • IL-7 receptor subunit alpha
    • IL-7R subunit alpha
    • IL-7R-alpha
    • IL-7RA
    • IL7R
    • IL7RA
    • IL7RA_HUMAN
    • IL7Ralpha
    • ILRA
    • Interleukin 7 receptor
    • Interleukin 7 receptor alpha chain
    • Interleukin 7 receptor isoform H5 6
    • Interleukin-7 receptor subunit alpha
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IL17R ELISA Kit (ab213799) Standard Curve

Protocols

References

ab213799 has not yet been referenced specifically in any publications.

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