Overview

  • Product name
    Human CD163 ELISA Kit (M130)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    0.032 ng/ml - 8 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 104.8 97% - 113%
    Plasma = 102.1 94% - 110%
    Cell culture media = 99.57 93% - 106%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Product overview

    Abcam’s CD163 (M130) Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human CD163 in serum, plasma (EDTA plasma is not suitable for use in this assay due to its chelating property) and cell culture supernatants.

    This assay employs an antibody specific for Human CD163 (M130) coated on a 96-well plate. Standards and samples are pipetted into the wells and CD163 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD163 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD163 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155428 - Assay Diluent A.

  • Representative standard curve using ab155428 -  Assay Diluent B

Protocols

References

ab155428 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer


Unfortunately, EDTA plasma is not suitable for use in this assay due to its chelating property.



I hope this helps!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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