Key features and details
- One-wash 90 minute protocol
- Sensitivity: 57 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: EDTA Plasma, Hep Plasma, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CD16b ELISA Kit
See all CD16b kits
Intra-assay Sample n Mean SD CV% Extract 24 3.1% Inter-assay Sample n Mean SD CV% Extract 3 3.2%
Sample typeTissue Extracts, Hep Plasma, EDTA Plasma
Assay typeSandwich (quantitative)
Range93.75 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Tissue Extracts 114 112% - 116% Hep Plasma 111 105% - 114% EDTA Plasma 114 110% - 116%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human CD16b ELISA Kit (ab253215) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD16b protein in hep plasma, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD16b with 57 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD16b, also known as FcγRIIIb, is a low affinity IgG receptor expressed on granulocytes. It is thought to be a dummy receptor for IgG’s to block destruction of the bound target by neutrophils. It is currently being investigated as a druggable-target to increase tumor cell destruction.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human CD16b Capture Antibody 1 x 600µl 10X Human CD16b Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human CD16b Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Information by UniProt
- CD16b antigen
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The CD16b standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of CD16b were measured in duplicates, interpolated from the CD16b standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD16b concentration was determined to be 270.43 ng/mL in plasma (EDTA), and 218.25 ng/mL in plasma (heparin).
The concentrations of CD16b were measured in duplicates, interpolated from the CD16b standard curves and corrected for sample dilution. Undiluted samples are as follows: human fetal spleen extracts 250 mg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD16b concentration was determined to be 4.37 ng/mL in and human fetal spleen supernatant.
ab253215 has not yet been referenced specifically in any publications.