Overview

  • Product name

    Human CD200 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    0.025 ng/ml - 6 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 86.14 77% - 95%
    Plasma = 78.35 67% - 86%
    Cell culture media = 81.85 74% - 89%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CD200 Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human CD200 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD200 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD200 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD200 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155429

Protocols

References

ab155429 has not yet been referenced specifically in any publications.

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