Overview

  • Product name

    Human CD244 ELISA Kit (2B4)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 0.45ng/ml 0.022 4.9%
    2 16 1.2ng/ml 0.076 6.3%
    3 16 3.2ng/ml 0.186 5.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 0.43ng/ml 0.027 6.2%
    2 24 1.3ng/ml 0.098 7.5%
    3 24 2.9ng/ml 0.194 6.7%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human CD244 Enzyme-Linked Immunosorbent Assay (ELISA) kit (2B4) (ab213758) is designed for the quantitative measurement of Human CD244 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD244/2B4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: C22-R221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD244/2B4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD244/2B4 amount of sample captured in plate.

  • Notes

    CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead, the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human CD244 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human CD244 antibody 1 x 130µl
    Lyophilized recombinant Human CD244 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Modulate other receptor-ligand interactions to enhance leukocyte activation.
  • Tissue specificity

    Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils.
  • Sequence similarities

    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • 2B4
    • C9.1
    • CD244
    • CD244 antigen
    • CD244 molecule
    • CD244 molecule natural killer cell receptor 2B4
    • CD244 natural killer cell receptor 2B4
    • CD244_HUMAN
    • F730046O15Rik
    • h2B4
    • Ly90
    • NAIL
    • Natural killer cell activation-inducing ligand
    • Natural killer cell receptor 2B4
    • NK cell activation inducing ligand
    • NK cell activation inducing ligand NAIL
    • NK cell activation-inducing ligand
    • NK cell type I receptor protein 2B4
    • NKR2B4
    • Nmrk
    • Non-MHC restricted killing associated
    • OTTHUMP00000027884
    • p38
    • signaling lymphocytic activation molecule 4
    • SLAM family, member 4
    • SLAMF4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human CD244 ELISA Kit (2B4) (ab213758) Standard Curve.

Protocols

References

ab213758 has not yet been referenced specifically in any publications.

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