Overview

  • Product name

    Human CD276 ELISA Kit
    See all CD276 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 9.9%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    178 pg/ml
  • Range

    578 pg/ml - 37000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94 83% - 101%
    Urine 89 88% - 91%
    Serum 102 100% - 104%
    Heparin Plasma 87 85% - 89%
    EDTA Plasma 91 87% - 94%
    Citrate Plasma 94 90% - 100%

  • Assay time

    2h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    CD276 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD276 protein in human serum, plasma, supernatant and urine.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    CD276 (B7-H3) is a glycoprotein member of the B7 family, Ig superfamily of proteins. CD276 is involved in the regulation of T-cell-mediated immune response and is expressed by a variety of cell types.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CD276 Capture Antibody 1 x 600µl
    10X Human CD276 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human CD276 Lyophilized Recombinant Protein 1 x 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NS 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
  • Tissue specificity

    Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • 4Ig B7 H3
    • 4Ig-B7-H3
    • AU016588
    • B7 H3
    • B7 homolog 3
    • B7-H3
    • B7H3
    • B7RP-2
    • CD_antigen=CD276
    • CD276
    • CD276 antigen
    • CD276 molecule
    • CD276_HUMAN
    • Costimulatory molecule
    • Flags: Precursor
    • PSEC0249
    • UNQ309/PRO352
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab221831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Interpolated concentrations of CD276 in human serum and plasma samples. The concentrations of CD276 were measured in duplicates, interpolated from the CD276 standard curves and corrected for sample dilution. Undiluted samples: serum 50%, plasma (EDTA) 25%, plasma (citrate) 25%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD276 concentration was determined to be 31.7 ng/mL in serum, 25.0 ng/mL in plasma (EDTA), 25.1 ng/mL in plasma (citrate) and 28.5 ng/mL in plasma (heparin).

  • Example of human CD276 standard curve in Sample Diluent NS.

Protocols

References

ab221831 has not yet been referenced specifically in any publications.

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