Overview

  • Product name

    Human CD31 ELISA Kit (PECAM)
    See all CD31 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell extract 8 3.4%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 3 3.3%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    44 pg/ml
  • Range

    226.6 pg/ml - 14500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 80 76% - 84%
    Serum 113 109% - 119%
    Heparin Plasma 74 71% - 80%
    EDTA Plasma 103 102% - 106%
    Citrate Plasma 91 88% - 95%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human, Rhesus monkey
    Does not react with: Mouse, Rat, Cow
  • Product overview

    As of March 1, 2019, Human CD31 SimpleStep ELISA® kit has been re-developed with new recombinant monoclonal antibodies that provide a higher sensitivity when quantifying CD31 protein in human serum, plasma, urine, saliva, cell culture supernatant, and cell and extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    CD31, also known as PECAM-1, is a cell adhesion molecule which is required for leukocyte trans-endothelial migration (TEM) under most inflammatory conditions. CD31 is a 130-kDa glycoprotein constitutively expressed on endothelial cells, platelets, neutrophils, monocytes, lymphocytes and basophils. CD31 molecules is expressed in large amounts in the intracellular junctions of endothelial cells. Experiments using antibody results in blocking CD31 homophilic interaction and inhibition of leukocytes transmigration. Expression of CD31 on the cell surface is regulated by receptor shedding resulting in generation of a soluble 100-kDa extracellular domain form and a truncated 28-kDa fragment containing the transmembrane and cytoplasmic domain of CD31.


     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab190814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between human CD31 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Standard Curve comparison between human CD31 new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.

  • The CD31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The CD31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

     

  • The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 36%, plasma (citrate) 9%, plasma (heparin) 9% and plasma (EDTA) 9%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.60 ng/mL in neat serum, 5.04 ng/mL in neat plasma (citrate), 5.67 ng/mL in neat plasma (heparin), and 6.44 ng/mL in neat plasma (EDTA).

  • THP-1 cells were cultured in the presence (stimulated) or absence (mock) of 5 µg/mL LPS and 50nM PMA for 24 hours. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 36%, urine 36%, THP-1 stimulated supernatant 18%, and THP-1 mock supernatant 18%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.85 ng/mL in neat saliva, 0.81 ng/mL in neat urine, 2.27 ng/mL in neat stimulated THP-1 supernatant, and 0.64 ng/mL in neat mock THP-1 supernatant.

  • THP-1 cells were cultured in the presence (stimulated) or absence (unstimulated) of 5 µg/mL LPS and 50nM PMA for 24 hours. The concentrations of CD31 were measured in duplicate and interpolated from the CD31 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 16.0 ng/mL in stimulated THP-1 cell extract and 12.5 ng/mL in unstimulated THP-1 cell extract.

     

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.34 ng/mL with a range of 4.39 – 11.1 ng/mL

Protocols

References

This product has been referenced in:

  • Meng D  et al. Effects of VEGFR1+ hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells. J Cancer Res Clin Oncol 145:411-427 (2019). Read more (PubMed: 30483898) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190814.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up