Overview

  • Product name

    Human CD31 ELISA Kit (PECAM1)
    See all CD31 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 113.8 105% - 121%
    Serum 106.1 95% - 114%
    Plasma 91.04 80% - 98%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CD31 (PECAM1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD31 (PECAM1) in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human CD31 (PECAM1) coated on a 96-well plate. Standards and samples are pipetted into the wells and CD31 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD31 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD31 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human CD31 ELISA Kit (PECAM) (ab190814) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100627.

  • Representative Standard Curve using ab100627.

Protocols

References

ab100627 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



Le confirmo que ambos kits detectan la parte soluble de PECAM1. La principal diferencia que presentan es la sensibilidad. ab45910 cubre el rango comprendido entre 0.31 ng/ml - 10 ng/ml, mientras que ab100627 es más sensible, abarcando el rango 8.23 pg/ml - 6000 pg/ml.

Dependiendo de la cantidad de PECAM1 que la usuaria quiera detectar en sus muestras, le será más conveniente una u otra opción.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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