Overview

  • Product name

    Human CD31 ELISA Kit (PECAM1)
    See all CD31 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample A 11 5.323ng/ml 0.19 3.5%
    Sample B 11 1.236ng/ml 0.077 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 11 5.406ng/ml 0.292 5.4%
    Sample B 11 1.205ng/ml 0.058 4.8%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.17 ng/ml
  • Range

    0.31 ng/ml - 10 ng/ml
  • Assay time

    2h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CD31 (PECAM1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 in Human serum, plasma, buffered solutions and cell culture medium


    A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD31 present in the samples.


    This kit will recognize both endogenous and recombinant Human CD31.


    Get results in 90 minutes with Human CD31 ELISA Kit (PECAM) (ab190814) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab45910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab45910.

References

This product has been referenced in:

  • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »
See 1 Publication for this product

Customer reviews and Q&As

Answer



Le confirmo que ambos kits detectan la parte soluble de PECAM1. La principal diferencia que presentan es la sensibilidad. ab45910 cubre el rango comprendido entre 0.31 ng/ml - 10 ng/ml, mientras que ab100627 es más sensible, abarcando el rango 8.23 pg/ml - 6000 pg/ml.

Dependiendo de la cantidad de PECAM1 que la usuaria quiera detectar en sus muestras, le será más conveniente una u otra opción.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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