Overview

  • Product name

    Human CD4 ELISA Kit
    See all CD4 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell extract 3 1.2%
    Inter-assay
    Sample n Mean SD CV%
    Cell extract 8 6.4%
  • Sample type

    Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    13.7 pg/ml
  • Range

    0.469 ng/ml - 30 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 100 96% - 105%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    CD4 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD4 protein in human cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of immune cells such as T helper, macrophages, monocytes and dendritic cells. CD4+ T cells (T helper cells) are lymphocytes essential in the function of a healthy human immune system. An instrumental role of the helper cell is the expression and secretion of cytokine signals to other cells, including CD8 cytotoxic T cells in order to mount an immune response. In HIV infected individuals, the virus is involved in the depletion of T helper cells making the individual vulnerable to otherwise innocuous pathogens.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CD4 Capture Antibody 1 x 600µl
    10X Human CD4 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human CD4 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Alternative names

    • CD 4
    • CD4
    • CD4 (L3T4)
    • CD4 antigen
    • CD4 antigen (p55)
    • CD4 molecule
    • CD4 receptor
    • CD4+ Lymphocyte deficiency, included
    • CD4_HUMAN
    • CD4mut
    • L3T4
    • Leu3
    • Ly-4
    • Lymphocyte antigen CD4
    • MGC165891
    • OTTHUMP00000238897
    • p55
    • T cell antigen T4
    • T cell antigen T4/LEU3
    • T cell differentiation antigen L3T4
    • T cell OKT4 deficiency, included
    • T cell surface antigen T4/Leu 3
    • T cell surface antigen T4/Leu3
    • T cell surface glycoprotein CD4
    • T-cell surface antigen T4/Leu-3
    • T-cell surface glycoprotein CD4
    • W3/25
    • W3/25 antigen
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab234569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD4 were measured in duplicate and interpolated from the CD4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD4 concentration was determined to be 8.95 ng/mL in HL-60 extract.

Protocols

References

ab234569 has not yet been referenced specifically in any publications.

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