Overview

  • Product name

    Human CD40 ELISA Kit
    See all CD40 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    54.87 pg/ml - 40000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.27 80% - 100%
    Serum 114.7 104% - 124%
    Plasma 90.19 78% - 100%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CD40 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Biotinylated anti-human CD40 2 vials
    CD40 Microplate (12 strips x 8 wells) 1 unit
    CD40 Standard Human (recombinant) (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
  • Alternative names

    • AI326936
    • B cell associated molecule
    • B cell associated molecule CD40
    • B cell surface antigen CD40
    • B cell-associated molecule
    • Bp50
    • CD 40
    • CD40 antigen
    • CD40 antigen (TNF receptor superfamily member 5)
    • CD40 molecule
    • CD40 molecule TNF receptor superfamily member 5
    • CD40 molecule, TNF receptor superfamily member 5
    • CD40 protein
    • CD40 type II isoform
    • CD40L receptor
    • CDw40
    • GP39
    • HIGM1
    • IGM
    • MGC9013
    • Nerve growth factor receptor related B lymphocyte activation molecule
    • NFRSF5
    • OTTHUMP00000031699
    • OTTHUMP00000031700
    • p50
    • T BAM
    • T-BAM
    • TBAM
    • TNF receptor superfamily member 5
    • TNFRSF5
    • TRAP
    • Tumor necrosis factor receptor superfamily member 5
    • Tumor necrosis factor receptor superfamily member 5 precursor
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99990

Protocols

References

This product has been referenced in:

  • Hosseinkhani B  et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). Read more (PubMed: 30131806) »
  • Xie JX  et al. Circulating CD40 and sCD40L Predict Changes in Renal Function in Subjects with Chronic Kidney Disease. Sci Rep 7:7942 (2017). Read more (PubMed: 28801616) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you very much for your interest in ab99990.


To our knowledge, ab99990 has not been tested in canine samples. Overall, there is only about 70% homology with the human and canine sequences. Therefore, I can offer a discount off a future purchase if you buy ab99990 now, test it incanine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: $470 off your next order

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab99990 in canine. I will then send a discount code. This code must be issued before purchasing ab99990 so please wait for my reply before ordering.

2. Purchase ab99990 either by phone, fax, or online (www.abcam.com).

3. Test it in canine.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for $470 and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab99990 turns out to be unsuitable for canine, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for your inquiry.


So I received a fairly cryptic reply from the lab regarding your inquiry. They said that upon request, they can sell antibodies from their kits separately, but since these are special requests, there won't be a whole lot of information on the datasheet for these products (i.e. immunogen). Plus, these antibodies are only evaluated in sELISA, and I think I recall you saying that you would like to use this in WB, which is untested and therefore not guaranteed to work in WB.


With all this in mind, let me know if you would like me to get the antibody added to the catalogue and I will follow up with the lab to get pricing/availability information. I look forward to your reply.

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