Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CD40L ELISA Kit
See all CD40L kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 6 pg/ml
Range8.23 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.35 86% - 105% Serum 93.57 83% - 103% Plasma 94.63 84% - 105%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human CD40L ELISA Kit (ab99991) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40L in serum, plasma (EDTA or heparin), and cell culture supernatants.
This assay employs an antibody specific for Human CD40L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human CD40 Ligand ELISA Kit (TNFSF5) (ab196268) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human CD40L (lyophilized) 2 vials CD40L Microplate (12 x 8 well strips) 1 unit Recombinant Human CD40L Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD40 antigen ligand
- CD40 ligand
SimpleStep ELISA kits
ab99991 has been referenced in 2 publications.
- Kuryk L et al. Quantification and functional evaluation of CD40L production from the adenovirus vector ONCOS-401. Cancer Gene Ther 26:26-31 (2019). PubMed: 30057416
- Garofalo M et al. Heterologous and cross-species tropism of cancer-derived extracellular vesicles. Theranostics 9:5681-5693 (2019). PubMed: 31534511