Human CD44 ELISA Kit (ab45912)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Human CD44 ELISA Kit
See all CD44 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample A 8 9.85ng/ml 1.55 1.52% Sample B 8 10.5ng/ml 0.055 4.76% Inter-assay Sample n Mean SD CV% Sample A 50 9.95ng/ml 0.307 3.08% Sample B 50 1.07ng/ml 0.105 9.34% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.13 ng/ml -
Range
0.31 ng/ml - 10 ng/ml -
Assay time
2h 10m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples.
This kit will recognize both endogenous and recombinant Human CD44.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-CD44 anitibody Red 1 x 0.3ml 2 x 0.3ml CD44 Control Silver 2 vials 4 vials CD44 Microplate (12 x 8 well strips) 1 unit 2 units CD44 Standard Yellow 2 vials 4 vials Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- LHR
- BA-1
- CD 44
see all -
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
Applications
Our Abpromise guarantee covers the use of ab45912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Dose Response Curve (Logarithmic) -
Sandwich ELISA - hu CD44 ELISA kit (ab45912)huCD44 measured in biofluids showing quantity (ng) per mL of tested sample
Protocols
References
This product has been referenced in:
- Lau WM et al. CD44v8-10 is a cancer-specific marker for gastric cancer stem cells. Cancer Res 74:2630-41 (2014). Read more (PubMed: 24618343) »
- Simpson KL et al. A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis 4:e613 (2013). ELISA ; Human . Read more (PubMed: 23640455) »
